EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.56 | D192A/S193A |
temperature-sensitive vD12 allele, near-lethality, only grows very slowly at 19°C |
677246 |
| 2.1.1.56 | D243A |
79% of wild-type methylation activity |
734586 |
| 2.1.1.56 | D243A |
the point mutation in the ExoN domain does not significantly interfere with the N7-MTase function |
706535 |
| 2.1.1.56 | D273A |
97% of wild-type methylation activity |
734586 |
| 2.1.1.56 | D330A |
the mutation does not affect viral replication, sensitivity to mutagen, or inhibition by interferon-beta compared to the wild type |
757520 |
| 2.1.1.56 | D331A |
complete loss of methylation activity |
734586 |
| 2.1.1.56 | D331A |
mutation abolishes the N7-MTase activity on GTP |
735335 |
| 2.1.1.56 | D331A |
the mutation destroys the MTase activity of nsp14 in vivo |
706535 |
| 2.1.1.56 | D331E |
70% of wild-type methylation activity |
734586 |
| 2.1.1.56 | D331Y |
complete loss of methylation activity |
734586 |
