EC Number |
Protein Variants |
Reference |
---|
2.1.1.179 | R181A |
tobramycin MIC is drastically reduced compared to wild-type enzyme |
712741 |
2.1.1.179 | K174A |
tobramycin MIC is drastically reduced compared to wild-type enzyme. 0.7% of the methylation activity compared to wild-type enzyme |
712741 |
2.1.1.179 | E182A |
tobramycin MIC is identical with that of wild-type RmtB |
712741 |
2.1.1.179 | R17A |
tobramycin MIC is identical with that of wild-type RmtB |
712741 |
2.1.1.179 | R48A |
tobramycin MIC is identical with that of wild-type RmtB |
712741 |
2.1.1.179 | Y56F |
tobramycin MIC is identical with that of wild-type RmtB |
712741 |
2.1.1.179 | S83A |
tobramycin MIC is identical with that of wild-type RmtB. 18% of the methylation activity compared to wild-type enzyme |
712741 |
2.1.1.179 | K14A |
tobramycin MIC is identical with that of wild-type RmtB. 36% of the methylation activity compared to wild-type enzyme |
712741 |
2.1.1.179 | H81A |
tobramycin MIC is identical with that of wild-type RmtB. No change in methylation activity compared to wild-type enzyme |
712741 |