2.1.1.179	additional information	analysis of sequence–function relationships of Sgm MTase by limited proteolysis and site-directed and random mutagenesis	702415	
2.1.1.179	additional information	construction of a series of in-frame knockout and knock-in mutants of Escherichia coli, corresponding to the genotypes rsmF+, DELTArsmF, rsmF+ rmtC+, and DELTArsmF rmtC+, mutant strain growth kinetics, overview	-, 718596	
2.1.1.179	H54A	inactive. Mutation dos not impact 30S binding affinity. Mutant strain is sensitive to kanamycin and gentamicin	757224	
2.1.1.179	H54E	inactive. Mutation dos not impact 30S binding affinity. Mutant strain is sensitive to kanamycin and gentamicin	757224	
2.1.1.179	E205A	mutant retains S-adenosyl-L-methionine binding	704280	
2.1.1.179	E267A	mutant retains S-adenosyl-L-methionine binding	704280	
2.1.1.179	K199A	mutant retains S-adenosyl-L-methionine binding	704280	
2.1.1.179	R236A	mutant retains S-adenosyl-L-methionine binding	704280	
2.1.1.179	D156A	mutant with drastically increased sensitivity to kanamycin	702415	
2.1.1.179	D158A	mutant with drastically increased sensitivity to kanamycin	702415	
2.1.1.179	D182A	mutant with drastically increased sensitivity to kanamycin	702415	
2.1.1.179	E205A	mutant with drastically increased sensitivity to kanamycin	702415	
2.1.1.179	E267A	mutant with drastically increased sensitivity to kanamycin	702415	
2.1.1.179	G135A	mutant with drastically increased sensitivity to kanamycin	702415	
2.1.1.179	K199A	mutant with drastically increased sensitivity to kanamycin	702415	
2.1.1.179	K54A	mutant with drastically increased sensitivity to kanamycin	702415	
2.1.1.179	R108A	mutant with drastically increased sensitivity to kanamycin	702415	
2.1.1.179	R187S	mutant with drastically increased sensitivity to kanamycin	702415	
2.1.1.179	R236A	mutant with drastically increased sensitivity to kanamycin	702415	
2.1.1.179	R433A	mutant with drastically increased sensitivity to kanamycin	702415	
2.1.1.179	R187S/G212S	mutant with strongly reduced activity	702415	
2.1.1.179	K20E	mutation eliminates 30S binding affinity, mutant strain is sensitive to kanamycin and gentamicin	757224	
2.1.1.179	R68E	mutation reduces 30S binding affinity about 11-13fold, resistance to kanamycin and gentamcin is reduced	757224	
2.1.1.179	R50E	mutation reduces 30S binding affinity about 11-13fold. Mutant strain is sensitive to kanamycin and gentamicin	757224	
2.1.1.179	K72E	mutation reduces 30S binding affinity about 5fold, resistance to kanamycin and gentamcin is reduced	757224	
2.1.1.179	D156A	no binding of S-adenosyl-L-methionine	704280	
2.1.1.179	D182A	no binding of S-adenosyl-L-methionine	704280	
2.1.1.179	G135A	no binding of S-adenosyl-L-methionine	704280	
2.1.1.179	additional information	replacement of the RmtC loop with four Ala residues (Loop237-246 ->A4) ablates the enzyme's ability to confer resistance to kanamycin and gentamicin. Conserved C-terminal domain residues surrounding the SAM-binding pocket are functionally critical but do not contribute to 30S binding affinity	757224	
2.1.1.179	H50A	tobramycin MIC is drastically reduced compared to wild-type enzyme	712741	
2.1.1.179	R181A	tobramycin MIC is drastically reduced compared to wild-type enzyme	712741	
2.1.1.179	K174A	tobramycin MIC is drastically reduced compared to wild-type enzyme. 0.7% of the methylation activity compared to wild-type enzyme	712741	
2.1.1.179	E182A	tobramycin MIC is identical with that of wild-type RmtB	712741	
2.1.1.179	R17A	tobramycin MIC is identical with that of wild-type RmtB	712741	
2.1.1.179	R48A	tobramycin MIC is identical with that of wild-type RmtB	712741	
2.1.1.179	Y56F	tobramycin MIC is identical with that of wild-type RmtB	712741	
2.1.1.179	S83A	tobramycin MIC is identical with that of wild-type RmtB. 18% of the methylation activity compared to wild-type enzyme	712741	
2.1.1.179	K14A	tobramycin MIC is identical with that of wild-type RmtB. 36% of the methylation activity compared to wild-type enzyme	712741	
2.1.1.179	H81A	tobramycin MIC is identical with that of wild-type RmtB. No change in methylation activity compared to wild-type enzyme	712741