EC Number   |
Protein Variants |
Reference |
|---|
    1.3.98.3 | H58L |
inactive mutant, no Fe-S cluster formation |
656073 |
| 1.3.98.3 | I329A |
contains the same amount of iron-sulfur cluster as the wild-type HemN, but cleaves only one S-adenosylmethionine molecule per molecule protein, no detectable CPO activity |
674462 |
| 1.3.98.3 | I329A |
exhibits the same yellow-brown color as wild-type HemN, but the [4Fe-4S] cluster content is slightly reduced and cleaves only one S-adenosylmethionine molecule per molecule protein, no detectable CPO activity |
674462 |
| 1.3.98.3 | more |
construction of deletion mutants of the HemN genes, growth of the mutant DELTAsll1876 is significantly slower than that of the wild-type under microoxic conditions, while it grows normally under aerobic conditions. Coproporphyrin III is accumulated at a low but significant level in the DELTAsll1876 mutant grown under micro-oxic conditions. No detectable phenotype in DELTAsll1917 under the conditions, phenotypes, overview |
713258 |
| 1.3.98.3 | more |
enzyme knockout by T-DNA insertions in the HEMN1 gene. A 17fold downregulation of AtHEMN1 transcripts occurs in the mutant. The plastidial CPO gene (LIN2) also shows 3.7fold downregulation. The AtHEMN1 mutation prevents the fusion of polar nuclei in the female gametophyte and affects endosperm proliferation. Phenotype, overview |
746121 |
| 1.3.98.3 | Q311A |
contains the same amount of iron-sulfur cluster as the wild-type HemN, but cleaves only one S-adenosylmethionine molecule per molecule protein, no detectable CPO activity |
674462 |
| 1.3.98.3 | Q311A |
exhibits the same yellow-brown color as wild-type HemN, but the [4Fe-4S] cluster content is slightly reduced and cleaves only one S-adenosylmethionine molecule per molecule protein, no detectable CPO activity |
674462 |
| 1.3.98.3 | Y56A |
appears colorless, the [4Fe-4S] cluster content is slightly reduced, no detectable S-adenosylmethionine cleavage, no detectable CPO activity |
674462 |
| 1.3.98.3 | Y56F |
mutant with 45% of wild-type activity and reduced Fe-S cluster formation |
656073 |
| 1.3.98.3 | Y56L |
appears colorless, the [4Fe-4S] cluster content is slightly reduced, no detectable S-adenosylmethionine cleavage, no detectable CPO activity |
674462 |
