1.3.98.3	C62S	inactive mutant, no Fe-S cluster formation	656073	
1.3.98.3	C66S	inactive mutant, no Fe-S cluster formation	656073	
1.3.98.3	C69S	inactive mutant, no Fe-S cluster formation	656073	
1.3.98.3	C71S	inactive mutant, same Fe-S cluster formation as in wild-type HemN	656073	
1.3.98.3	E145A	appears colorless, the [4Fe-4S] cluster content is slightly reduced, no detectable S-adenosylmethionine cleavage, no detectable CPO activity	674462	
1.3.98.3	E145I	appears colorless, the [4Fe-4S] cluster content is slightly reduced, no detectable S-adenosylmethionine cleavage, no detectable CPO activity	674462	
1.3.98.3	F310A	is slightly yellow, the [4Fe-4S] cluster content is slightly reduced, cleaves only one S-adenosylmethionine molecule per molecule protein, residual CPO activity	674462	
1.3.98.3	F310L	is slightly yellow, the [4Fe-4S] cluster content is slightly reduced, cleaves only one S-adenosylmethionine molecule per molecule protein, no detectable CPO activity	674462	
1.3.98.3	F68L	mutant with 89% of wild-type activity	656073	
1.3.98.3	G111V/G113V	double mutation of Gly-111 and Gly-113, which are part of the potential GGGTP S-adenosyl-L-methionine binding motif, completely abolishes enzyme activity, reduced Fe-S cluster formation	656073	
1.3.98.3	H58L	inactive mutant, no Fe-S cluster formation	656073	
1.3.98.3	I329A	contains the same amount of iron-sulfur cluster as the wild-type HemN, but cleaves only one S-adenosylmethionine molecule per molecule protein, no detectable CPO activity	674462	
1.3.98.3	I329A	exhibits the same yellow-brown color as wild-type HemN, but the [4Fe-4S] cluster content is slightly reduced and cleaves only one S-adenosylmethionine molecule per molecule protein, no detectable CPO activity	674462	
1.3.98.3	additional information	construction of deletion mutants of the HemN genes, growth of the mutant DELTAsll1876 is significantly slower than that of the wild-type under microoxic conditions, while it grows normally under aerobic conditions. Coproporphyrin III is accumulated at a low but significant level in the DELTAsll1876 mutant grown under micro-oxic conditions. No detectable phenotype in DELTAsll1917 under the conditions, phenotypes, overview	713258	
1.3.98.3	additional information	enzyme knockout by T-DNA insertions in the HEMN1 gene. A 17fold downregulation of AtHEMN1 transcripts occurs in the mutant. The plastidial CPO gene (LIN2) also shows 3.7fold downregulation. The AtHEMN1 mutation prevents the fusion of polar nuclei in the female gametophyte and affects endosperm proliferation. Phenotype, overview	746121	
1.3.98.3	Q311A	contains the same amount of iron-sulfur cluster as the wild-type HemN, but cleaves only one S-adenosylmethionine molecule per molecule protein, no detectable CPO activity	674462	
1.3.98.3	Q311A	exhibits the same yellow-brown color as wild-type HemN, but the [4Fe-4S] cluster content is slightly reduced and cleaves only one S-adenosylmethionine molecule per molecule protein, no detectable CPO activity	674462	
1.3.98.3	Y56A	appears colorless, the [4Fe-4S] cluster content is slightly reduced, no detectable S-adenosylmethionine cleavage, no detectable CPO activity	674462	
1.3.98.3	Y56F	mutant with 45% of wild-type activity and reduced Fe-S cluster formation	656073	
1.3.98.3	Y56L	appears colorless, the [4Fe-4S] cluster content is slightly reduced, no detectable S-adenosylmethionine cleavage, no detectable CPO activity	674462	
1.3.98.3	Y74F	the apparent binding affinity of the mutant enzyme for the cofactor S-adenosyl-L-methionine is higher than that of the wild type enzyme	-, 739104