EC Number   |
Cofactor |
Reference |
|---|
    2.8.1.6 | iron-sulfur centre |
- |
702042, 702256, 702281, 704715 |
| 2.8.1.6 | NADH |
- |
645611 |
| 2.8.1.6 | NADPH |
- |
645582, 645588, 645605, 645611 |
| 2.8.1.6 | S-adenosyl-L-methionine |
- |
702042, 702256, 702281, 704715 |
| 2.8.1.6 | [2Fe-2S]-center |
a reaction intermediate includes Arg260 as a deprotonated guanidino group, coordinated to the ferrous iron of the auxiliary [2Fe-2S] cluster as a monodentate ligand. The 9-mercaptodethiobiotin C9 is covalently coordinated to the auxiliary [2Fe-2S] cluster, which maintains the geometric and electronic structure characteristics of a typical reduced [2Fe-2S]+ cluster, with most of the spin density localized on the cluster and only a small positive spin density residing on the 9-mercaptodethiobiotin ligand via through-bond (C9 and my-thiolate) spin delocalization. C6, the target of the second hydrogen-atom abstraction, is now located in close proximity to the newly acquired thiolate sulfur |
761350 |
| 2.8.1.6 | FAD |
enhances activity |
645589 |
| 2.8.1.6 | NADH |
enhances activity |
645589 |
| 2.8.1.6 | NADPH |
enhances activity |
645589, 645593 |
| 2.8.1.6 | more |
not: pyridoxal 5-phosphate |
661114 |
| 2.8.1.6 | NADPH |
omission results in a 100fold decrease in activity |
645595 |
