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Information on EC 2.8.1.6 - biotin synthase

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.1 Sulfurtransferases
                2.8.1.6 biotin synthase
IUBMB Comments
The enzyme binds a [4Fe-4S] and a [2Fe-2S] cluster. In every reaction cycle, the enzyme consumes two molecules of AdoMet, each producing 5'-deoxyadenosine and a putative dethiobiotinyl carbon radical. Reaction with another equivalent of AdoMet results in abstraction of the C6 methylene pro-S hydrogen atom from 9-mercaptodethiobiotin, and the resulting carbon radical is quenched via formation of an intramolecular C-S bond, thus closing the biotin thiophane ring. The sulfur donor is believed to be the [2Fe-2S] cluster, which is sacrificed in the process, so that in vitro the reaction is a single turnover. In vivo, the [2Fe-2S] cluster can be reassembled by the Isc or Suf iron-sulfur cluster assembly systems, to allow further catalysis.
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Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
Bio2, BIO2 protein (Arabidopsis thaliana clone pMP101 gene BIO2 reduced), BioB, biotin synthase, biotin synthase (Saccharomyces cerevisiae strain 20B-12 clone pUCH2.4 gene BIO2), biotin synthase (Treponema pallidum gene TP0228), biotin synthetase, biotin synthetase (Aquifex aeolicus gene bioB), biotin synthetase (Arabidopsis thaliana clone pMB101 gene BIO2 reduced), biotin synthetase (Bacillus subtilis gene bioB), more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin
show the reaction diagram
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