Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Lysinibacillus sphaericus | |
L-cysteine | - |
Lysinibacillus sphaericus | |
S-adenosyl-L-methionine | absolute requirement as electron source | Lysinibacillus sphaericus |
General Stability | Organism |
---|---|
loses all its clusters if the purification is carried out with buffers that have not been saturated with argon | Lysinibacillus sphaericus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | above 1 mM | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
dethiobiotin | - |
Lysinibacillus sphaericus | |
0.005 | - |
9-Mercaptodethiobiotin | - |
Lysinibacillus sphaericus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | presence of a [2Fe-2S] cluster | Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36954 | - |
2 * 36954, calculation from nucleotide sequence | Lysinibacillus sphaericus |
37000 | - |
2 * 37000, SDS-PAGE | Lysinibacillus sphaericus |
75000 | - |
gel filtration | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxygen sensitive | Lysinibacillus sphaericus |
Purification (Comment) | Organism |
---|---|
- |
Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9-mercaptodethiobiotin + sulfur | - |
Lysinibacillus sphaericus | biotin | - |
? | |
dethiobiotin + sulfur | ultimate sulfur donor has not yet been identified | Lysinibacillus sphaericus | biotin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Lysinibacillus sphaericus |
dimer | 2 * 36954, calculation from nucleotide sequence | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Lysinibacillus sphaericus |