EC Number |
pH Minimum |
pH Maximum |
Reference |
---|
2.5.1.47 | -999 |
- |
altered by immobilization |
637362 |
2.5.1.47 | -999 |
- |
pH-rate profiles for wild type and H152A mutant with O3-acetyl-L-serine and 5-thio-2-nitrobenzoate as substrates are similar, 100 mM concentrations of 2-(N-morpholino)ethanesulfonic acid (MES), pH 5.5-6.5, 3-(N-morpholino)propanesulfonic acid (MOPS), pH 7.0-7.5, N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid (HEPES), pH 8.0, and 3-[[tris(hydroxymethyl)]amino]propanesulfonic acid (TAPS), pH 9.0, titrated with KOH |
684707 |
2.5.1.47 | -999 |
- |
pH-rate profiles for wild type and H152A mutant with O3-acetyl-L-serine and beta-chloro-L-alanine as substrates are similar, 100 mM concentrations of 2-(N-morpholino)ethanesulfonic acid (MES), pH 5.5-6.5, 3-(N-morpholino)propanesulfonic acid (MOPS), pH 7.0-7.5, N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid (HEPES), pH 8.0, and 3-[[tris(hydroxymethyl)]amino]propanesulfonic acid (TAPS), pH 9.0, titrated with KOH |
685397 |
2.5.1.47 | 5 |
6.5 |
- |
759669 |
2.5.1.47 | 5.5 |
7.5 |
- |
759670 |
2.5.1.47 | 5.5 |
8 |
about 50% of maximal activity at pH 5.5 and pH 8.0 |
637383 |
2.5.1.47 | 5.8 |
9.4 |
- |
637328 |
2.5.1.47 | 6 |
10 |
pH 6: about 55% of maximal activity, pH 10.0: about 40% of maximal activity |
676961 |
2.5.1.47 | 7.3 |
8.2 |
isoenzyme 2 |
637341 |
2.5.1.47 | 8 |
9 |
- |
706226 |