Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.47 extracted from

  • Zhao, C.; Kumada, Y.; Imanaka, H.; Imamura, K.; Nakanishi, K.
    Cloning, overexpression, purification, and characterization of O-acetylserine sulfhydrylase-B from Escherichia coli (2006), Protein Expr. Purif., 47, 607-613.
    View publication on PubMed
Search for more literature for 2.5.1.47

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli using pUC19 with a lacUV5 promoter Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(NH4)6Mo7O24 1 mM, 61% loss of activity Escherichia coli
AgNO3 1 mM, complete loss of activity Escherichia coli
CuSO4 1 mM, 99% loss of activity Escherichia coli
FeSO4 1 mM, 99% loss of activity Escherichia coli
iodoacetamide 1 mM, 48% loss of activity Escherichia coli
PCMB 1 mM, 97% loss of activity Escherichia coli
ZnCl2 1 mM, 88% loss of activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.133
-
 sodium thiosulfate
-
 Escherichia coli
1.3
-
 O-acetyl-L-Ser
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32600
-
 2 * 32600, SDS-PAGE Escherichia coli
64000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
728
-
 25°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-Ser + 1,2,3,4-tetrazole
-
 Escherichia coli ?
-
 ?
O-acetyl-L-Ser + 1,2,3-benzotriazole weak activity Escherichia coli ?
-
 ?
O-acetyl-L-Ser + 1,2,4-triazole
-
 Escherichia coli ?
-
 ?
O-acetyl-L-Ser + 1-propanethiol
-
 Escherichia coli ?
-
 ?
O-acetyl-L-Ser + 3-mercapto-1,2,4-triazole
-
 Escherichia coli ?
-
 ?
O-acetyl-L-Ser + benzenethiol
-
 Escherichia coli L-cys + benzylacetate
-
 ?
O-acetyl-L-Ser + cysteamine weak activity Escherichia coli ?
-
 ?
O-acetyl-L-Ser + H2S
-
 Escherichia coli L-Cys + acetate
-
 ?
O-acetyl-L-Ser + pyrazole weak activity Escherichia coli ?
-
 ?
O-acetyl-L-Ser + sodium azide weak activity Escherichia coli ?
-
 ?
O-acetyl-L-Ser + sodium thiosulfate
-
 Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32600, SDS-PAGE Escherichia coli
More OASS-B does not form a complex with Escherichia coli serine acetyltransferase (SAT, EC 2.3.1.30) Escherichia coli

Synonyms

Synonyms Comment Organism
O-acetylserine sulfhydrylase-B
-
 Escherichia coli
OASS-B
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 60 1 h, pH 7.5, stable Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 9
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6: about 55% of maximal activity, pH 10.0: about 40% of maximal activity Escherichia coli