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EC Number	pH Optimum Minimum	pH Optimum Maximum	Commentary	Reference
2.4.2.10	-999	-	2 isozymes: pI: 5.65 (minor form), pI: 5.85 (major form)	489713
2.4.2.10	5.5	6.5	reverse (pyrophosphorolysis) reaction	687400
2.4.2.10	7	7.5	preferred buffer: Tris-HCl, when substrates are non-saturating, phosphate buffer, when substrate are saturating, not: maleate, imidazole, N-tris[hydroxymethyl] methyl-2-aminoethane-sulfonic acid (i.e. TES) or 3-[N-morpholino]-2-hydroxypropanesulfonic acid (i.e. MOPS) buffer	489712
2.4.2.10	7.4	-	assay at	676270
2.4.2.10	7.5	-	assay at	674045
2.4.2.10	7.6	-	assay at	673097
2.4.2.10	7.8	-	assay at	672162
2.4.2.10	8	-	-	489725
2.4.2.10	8	-	assay at	672923
2.4.2.10	8.5	-	mutant	489719

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Release 2023.1 (January 2023)