EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    4.1.1.72 | 0.09 |
- |
pyruvate |
pH 7.0, 55°C |
747761 |
| 4.1.1.72 | 0.17 |
- |
4-methyl-2-oxopentanoate |
pH 7.0, 55°C |
747761 |
| 4.1.1.72 | 0.19 |
- |
2-oxoisovalerate |
wild-type, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction |
656230 |
| 4.1.1.72 | 0.19 |
- |
2-oxoisovalerate |
wild-type, pH 7.5, 30°C, overall reaction |
656230 |
| 4.1.1.72 | 0.19 |
- |
thiamine diphosphate |
wild-type, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction |
656230 |
| 4.1.1.72 | 0.19 |
- |
thiamine diphosphate |
wild-type, pH 7.5, 30°C, overall reaction |
656230 |
| 4.1.1.72 | 0.3 |
- |
3-methyl-2-oxobutanoate |
pH 7.0, 55°C |
747761 |
| 4.1.1.72 | 0.31 |
- |
thiamine diphosphate |
mutant Y300A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction |
656230 |
| 4.1.1.72 | 0.31 |
- |
thiamine diphosphate |
mutant Y300A, pH 7.5, 30°C, overall reaction |
656230 |
| 4.1.1.72 | 0.38 |
- |
2-oxoisovalerate |
mutant Y300A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction |
656230 |
