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Literature summary for 4.1.1.72 extracted from

  • Heath, C.; Jeffries, A.; Hough, D.; Danson, M.
    Discovery of the catalytic function of a putative 2-oxoacid dehydrogenase multienzyme complex in the thermophilic archaeon Thermoplasma acidophilum (2004), FEBS Lett., 577, 523-527 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
3-methyl-2-oxobutanoate pH 7.0, 55°C Thermoplasma acidophilum
0.042
-
4-methyl-2-oxopentanoate pH 7.0, 55°C Thermoplasma acidophilum
0.1
-
3-methyl-2-oxopentanoate pH 7.0, 55°C Thermoplasma acidophilum
0.6
-
pyruvate pH 7.0, 55°C Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
165000
-
gel filtration Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum Q9HIA4 AND Q9HIA3 Q9HIA4: chain E1-beta, Q9HIA3: chain E1-alpha
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-2-oxobutanoate
-
Thermoplasma acidophilum 2-methylpropanal + CO2
-
?
3-methyl-2-oxopentanoate
-
Thermoplasma acidophilum 2-methylbutanal + CO2
-
?
4-methyl-2-oxopentanoate
-
Thermoplasma acidophilum 4-methylbutanal + CO2
-
?
additional information no activity with 2-oxoglutarate Thermoplasma acidophilum ?
-
?
pyruvate
-
Thermoplasma acidophilum acetaldehyde + CO2
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 43000 (alpha) + 2 * 37000 (beta), SDS-PAGE Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
assay at Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.09
-
pyruvate pH 7.0, 55°C Thermoplasma acidophilum
0.17
-
4-methyl-2-oxopentanoate pH 7.0, 55°C Thermoplasma acidophilum
0.3
-
3-methyl-2-oxobutanoate pH 7.0, 55°C Thermoplasma acidophilum
0.5
-
3-methyl-2-oxopentanoate pH 7.0, 55°C Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Thermoplasma acidophilum

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate required Thermoplasma acidophilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.15
-
pyruvate pH 7.0, 55°C Thermoplasma acidophilum
4
-
4-methyl-2-oxopentanoate pH 7.0, 55°C Thermoplasma acidophilum
5
-
3-methyl-2-oxopentanoate pH 7.0, 55°C Thermoplasma acidophilum
11
-
3-methyl-2-oxobutanoate pH 7.0, 55°C Thermoplasma acidophilum