Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
3-methyl-2-oxobutanoate | pH 7.0, 55°C | Thermoplasma acidophilum | |
0.042 | - |
4-methyl-2-oxopentanoate | pH 7.0, 55°C | Thermoplasma acidophilum | |
0.1 | - |
3-methyl-2-oxopentanoate | pH 7.0, 55°C | Thermoplasma acidophilum | |
0.6 | - |
pyruvate | pH 7.0, 55°C | Thermoplasma acidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
165000 | - |
gel filtration | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | Q9HIA4 AND Q9HIA3 | Q9HIA4: chain E1-beta, Q9HIA3: chain E1-alpha | - |
Purification (Comment) | Organism |
---|---|
- |
Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-oxobutanoate | - |
Thermoplasma acidophilum | 2-methylpropanal + CO2 | - |
? | |
3-methyl-2-oxopentanoate | - |
Thermoplasma acidophilum | 2-methylbutanal + CO2 | - |
? | |
4-methyl-2-oxopentanoate | - |
Thermoplasma acidophilum | 4-methylbutanal + CO2 | - |
? | |
additional information | no activity with 2-oxoglutarate | Thermoplasma acidophilum | ? | - |
? | |
pyruvate | - |
Thermoplasma acidophilum | acetaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 43000 (alpha) + 2 * 37000 (beta), SDS-PAGE | Thermoplasma acidophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Thermoplasma acidophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
pyruvate | pH 7.0, 55°C | Thermoplasma acidophilum | |
0.17 | - |
4-methyl-2-oxopentanoate | pH 7.0, 55°C | Thermoplasma acidophilum | |
0.3 | - |
3-methyl-2-oxobutanoate | pH 7.0, 55°C | Thermoplasma acidophilum | |
0.5 | - |
3-methyl-2-oxopentanoate | pH 7.0, 55°C | Thermoplasma acidophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Thermoplasma acidophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | required | Thermoplasma acidophilum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
pyruvate | pH 7.0, 55°C | Thermoplasma acidophilum | |
4 | - |
4-methyl-2-oxopentanoate | pH 7.0, 55°C | Thermoplasma acidophilum | |
5 | - |
3-methyl-2-oxopentanoate | pH 7.0, 55°C | Thermoplasma acidophilum | |
11 | - |
3-methyl-2-oxobutanoate | pH 7.0, 55°C | Thermoplasma acidophilum |