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Literature summary for 4.1.1.72 extracted from

  • Li, J.; Wynn, R.M.; Machius, M.; Chuang, J.L.; Karthikeyan, S.; Tomchick, D.R.; Chuang, D.T.
    Cross-talk between thiamin diphosphate binding and phosphorylation loop conformation in human branched-chain alpha-keto acid decarboxylase/dehydrogenase (2004), J. Biol. Chem., 279, 32968-32978.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D295A of component E1b, 2fold increase in kcat-value Homo sapiens
R287A of component E1b, 40fold increase in Km-value for 2-oxoisovalerate Homo sapiens
R301A of component E1b, 4fold increase in Km-value for 2-oxoisovalerate Homo sapiens
Y300A of component E1b, 6fold increase in Km-value for 2-oxoisovalerate Homo sapiens
Y300F of component E1b, 5fold increase in Km-value for 2-oxoisovalerate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00063
-
thiamine diphosphate wild-type, pH 7.5, 30°C, overall reaction Homo sapiens
0.0066
-
thiamine diphosphate wild-type, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.015
-
thiamine diphosphate mutant D295A, pH 7.5, 30°C, overall reaction Homo sapiens
0.016
-
thiamine diphosphate mutant D295A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.018
-
thiamine diphosphate mutant Y300F, pH 7.5, 30°C, overall reaction Homo sapiens
0.026
-
thiamine diphosphate mutant R301A, pH 7.5, 30°C, overall reaction Homo sapiens
0.026
-
thiamine diphosphate mutant Y300A, pH 7.5, 30°C, overall reaction Homo sapiens
0.028
-
thiamine diphosphate mutant Y300F, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.029
-
thiamine diphosphate mutant R301A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.04
-
thiamine diphosphate mutant Y300A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.05
-
2-oxoisovalerate mutant D295A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.05
-
2-oxoisovalerate mutant D295A, pH 7.5, 30°C, overall reaction Homo sapiens
0.05
-
2-oxoisovalerate wild-type, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.05
-
2-oxoisovalerate wild-type, pH 7.5, 30°C, overall reaction Homo sapiens
0.14
-
2-oxoisovalerate mutant Y300A, pH 7.5, 30°C, overall reaction Homo sapiens
0.225
-
2-oxoisovalerate mutant R301A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.252
-
2-oxoisovalerate mutant Y300F, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.255
-
2-oxoisovalerate mutant R301A, pH 7.5, 30°C, overall reaction Homo sapiens
0.337
-
2-oxoisovalerate mutant Y300A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.39
-
2-oxoisovalerate mutant Y300F, pH 7.5, 30°C, overall reaction Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(3S)-3-methyl-2-oxopentanoate = 2-methylbutanal + CO2 binding of cofactor thiamine diphosphate to E1b component of enzyme induces a disorder-to-order transition of the conserved phosphorylation loop carrying phosphorylation sites S292 and S302 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoisovalerate
-
Homo sapiens 2-methylpropanal + CO2
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.19
-
thiamine diphosphate wild-type, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.19
-
2-oxoisovalerate wild-type, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.19
-
thiamine diphosphate wild-type, pH 7.5, 30°C, overall reaction Homo sapiens
0.19
-
2-oxoisovalerate wild-type, pH 7.5, 30°C, overall reaction Homo sapiens
0.31
-
thiamine diphosphate mutant Y300A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.31
-
thiamine diphosphate mutant Y300A, pH 7.5, 30°C, overall reaction Homo sapiens
0.38
-
2-oxoisovalerate mutant Y300A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.38
-
2-oxoisovalerate mutant Y300A, pH 7.5, 30°C, overall reaction Homo sapiens
0.41
-
thiamine diphosphate mutant R301A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.41
-
2-oxoisovalerate mutant R301A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.41
-
thiamine diphosphate mutant R301A, pH 7.5, 30°C, overall reaction Homo sapiens
0.41
-
2-oxoisovalerate mutant R301A, pH 7.5, 30°C, overall reaction Homo sapiens
0.42
-
thiamine diphosphate mutant D295A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.42
-
thiamine diphosphate mutant D295A, pH 7.5, 30°C, overall reaction Homo sapiens
0.48
-
2-oxoisovalerate mutant D295A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.48
-
2-oxoisovalerate mutant D295A, pH 7.5, 30°C, overall reaction Homo sapiens
0.53
-
thiamine diphosphate mutant Y300F, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.53
-
thiamine diphosphate mutant Y300F, pH 7.5, 30°C, overall reaction Homo sapiens
0.57
-
2-oxoisovalerate mutant Y300F, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction Homo sapiens
0.57
-
2-oxoisovalerate mutant Y300F, pH 7.5, 30°C, overall reaction Homo sapiens

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate binds to E1b component of enzyme complex Homo sapiens