Protein Variants | Comment | Organism |
---|---|---|
D295A | of component E1b, 2fold increase in kcat-value | Homo sapiens |
R287A | of component E1b, 40fold increase in Km-value for 2-oxoisovalerate | Homo sapiens |
R301A | of component E1b, 4fold increase in Km-value for 2-oxoisovalerate | Homo sapiens |
Y300A | of component E1b, 6fold increase in Km-value for 2-oxoisovalerate | Homo sapiens |
Y300F | of component E1b, 5fold increase in Km-value for 2-oxoisovalerate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00063 | - |
thiamine diphosphate | wild-type, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.0066 | - |
thiamine diphosphate | wild-type, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.015 | - |
thiamine diphosphate | mutant D295A, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.016 | - |
thiamine diphosphate | mutant D295A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.018 | - |
thiamine diphosphate | mutant Y300F, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.026 | - |
thiamine diphosphate | mutant R301A, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.026 | - |
thiamine diphosphate | mutant Y300A, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.028 | - |
thiamine diphosphate | mutant Y300F, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.029 | - |
thiamine diphosphate | mutant R301A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.04 | - |
thiamine diphosphate | mutant Y300A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.05 | - |
2-oxoisovalerate | mutant D295A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.05 | - |
2-oxoisovalerate | mutant D295A, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.05 | - |
2-oxoisovalerate | wild-type, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.05 | - |
2-oxoisovalerate | wild-type, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.14 | - |
2-oxoisovalerate | mutant Y300A, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.225 | - |
2-oxoisovalerate | mutant R301A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.252 | - |
2-oxoisovalerate | mutant Y300F, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.255 | - |
2-oxoisovalerate | mutant R301A, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.337 | - |
2-oxoisovalerate | mutant Y300A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.39 | - |
2-oxoisovalerate | mutant Y300F, pH 7.5, 30°C, overall reaction | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(3S)-3-methyl-2-oxopentanoate = 2-methylbutanal + CO2 | binding of cofactor thiamine diphosphate to E1b component of enzyme induces a disorder-to-order transition of the conserved phosphorylation loop carrying phosphorylation sites S292 and S302 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoisovalerate | - |
Homo sapiens | 2-methylpropanal + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
thiamine diphosphate | wild-type, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.19 | - |
2-oxoisovalerate | wild-type, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.19 | - |
thiamine diphosphate | wild-type, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.19 | - |
2-oxoisovalerate | wild-type, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.31 | - |
thiamine diphosphate | mutant Y300A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.31 | - |
thiamine diphosphate | mutant Y300A, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.38 | - |
2-oxoisovalerate | mutant Y300A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.38 | - |
2-oxoisovalerate | mutant Y300A, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.41 | - |
thiamine diphosphate | mutant R301A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.41 | - |
2-oxoisovalerate | mutant R301A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.41 | - |
thiamine diphosphate | mutant R301A, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.41 | - |
2-oxoisovalerate | mutant R301A, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.42 | - |
thiamine diphosphate | mutant D295A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.42 | - |
thiamine diphosphate | mutant D295A, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.48 | - |
2-oxoisovalerate | mutant D295A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.48 | - |
2-oxoisovalerate | mutant D295A, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.53 | - |
thiamine diphosphate | mutant Y300F, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.53 | - |
thiamine diphosphate | mutant Y300F, pH 7.5, 30°C, overall reaction | Homo sapiens | |
0.57 | - |
2-oxoisovalerate | mutant Y300F, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction | Homo sapiens | |
0.57 | - |
2-oxoisovalerate | mutant Y300F, pH 7.5, 30°C, overall reaction | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | binds to E1b component of enzyme complex | Homo sapiens |