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EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Commentary	Reference
5.4.99.1	3.19	-	L-glutamate	23°C, mutant enzyme E171D	650824
5.4.99.1	5.4	-	L-threo-3-methylaspartate	wild-type, pH 7.0	677821
5.4.99.1	5.8	-	(2S,3S)-3-methylaspartate	genetically engineered enzyme with S subunit fused to the C-terminus of the E subunit through an 11 amino acid (Gly-Gln)5-Gly linker segment	3455
5.4.99.1	5.8	-	L-Glu	genetically engineered enzyme with S subunit fused to the C-terminus of the E subunit through an 11 amino acid (Gly-Gln)5-Gly linker segment	3455
5.4.99.1	5.8	-	L-glutamate	23°C, wild-type enzyme	650824
5.4.99.1	5.8	-	L-glutamate	25°C, wild-type enzyme	650247
5.4.99.1	20	-	L-Glu	reaction of L-Glu with benzimidazolribofuranosyl-adenosylcobinamide, wild-type enzyme	3458

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Release 2023.1 (January 2023)