Protein Variants | Comment | Organism |
---|---|---|
E171A | turnover number for glutamate is reduced 27.6fold, KM-value is increased 1.1fold, Km-value for adenosylcobalamin is reduced 1.23fold | Clostridium cochlearium |
E171D | turnover number for glutamate is reduced 1.8fold, KM-value is reduced 1.54fold, Km-value for adenosylcobalamin is reduced 2.7fold | Clostridium cochlearium |
E171N | turnover number for glutamate is reduced 232fold, KM-value is increased by 1.8fold, Km-value for adenosylcobalamin is reduced 1.4fold | Clostridium cochlearium |
E171Q | turnover number for glutamate is reduced 53fold, KM-value is reduced 2.4fold, Km-value for adenosylcobalamin is reduced 2fold, mutant enzyme is independent of pH | Clostridium cochlearium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
L-glutamate | 23°C, mutant enzyme E171Q | Clostridium cochlearium | |
0.38 | - |
L-glutamate | 23°C, mutant enzyme E171D | Clostridium cochlearium | |
0.58 | - |
L-glutamate | 23°C, wild-type enzyme | Clostridium cochlearium | |
0.65 | - |
L-glutamate | 23°C, mutant enzyme E171A | Clostridium cochlearium | |
1.07 | - |
L-glutamate | 23°C, mutant enzyme E171N | Clostridium cochlearium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium cochlearium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Clostridium cochlearium | L-threo-3-methylaspartate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
L-glutamate | 23°C, mutant enzyme E171N | Clostridium cochlearium | |
0.11 | - |
L-glutamate | 23°C, mutant enzyme E171Q | Clostridium cochlearium | |
0.21 | - |
L-glutamate | 23°C, mutant enzyme E171A | Clostridium cochlearium | |
3 | 6 | L-glutamate | 23°C, mutant enzyme E171D | Clostridium cochlearium | |
3.19 | - |
L-glutamate | 23°C, mutant enzyme E171D | Clostridium cochlearium | |
5.8 | - |
L-glutamate | 23°C, wild-type enzyme | Clostridium cochlearium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Clostridium cochlearium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.7 | pH 6.5: about 50% of maximal activity, pH 8.7: about 80% of maximal activity | Clostridium cochlearium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adenosylcobalamin | enzyme is dependent on, KM for wild-type enzyme is 0.0055 mM | Clostridium cochlearium |