5.4.99.1 0.0181 - L-glutamate 25°C, mutant enzyme R100Y 650247 5.4.99.1 0.0211 - L-glutamate 25°C, mutant enzyme R100M 650247 5.4.99.1 0.025 - L-glutamate 23°C, mutant enzyme E171N 650824 5.4.99.1 0.048 - L-glutamate 25°C, mutant enzyme R100K 650247 5.4.99.1 0.05 - L-2-hydroxyglutarate pH 7.0 649913 5.4.99.1 0.073 - L-threo-3-methylaspartate mutant R100K, pH 7.0 677821 5.4.99.1 0.11 - L-glutamate 23°C, mutant enzyme E171Q 650824 5.4.99.1 0.21 - L-glutamate 23°C, mutant enzyme E171A 650824 5.4.99.1 0.65 - L-Glu reaction of L-Glu with benzimidazolribofuranosyl-adenosylcobinamide, fusion protein in which the cobalamin-binding subunit is linked to the catalytic subunit 3458 5.4.99.1 3 6 L-glutamate 23°C, mutant enzyme E171D 650824 5.4.99.1 3.19 - L-glutamate 23°C, mutant enzyme E171D 650824 5.4.99.1 5.4 - L-threo-3-methylaspartate wild-type, pH 7.0 677821 5.4.99.1 5.8 - (2S,3S)-3-methylaspartate genetically engineered enzyme with S subunit fused to the C-terminus of the E subunit through an 11 amino acid (Gly-Gln)5-Gly linker segment 3455 5.4.99.1 5.8 - L-Glu genetically engineered enzyme with S subunit fused to the C-terminus of the E subunit through an 11 amino acid (Gly-Gln)5-Gly linker segment 3455 5.4.99.1 5.8 - L-glutamate 23°C, wild-type enzyme 650824 5.4.99.1 5.8 - L-glutamate 25°C, wild-type enzyme 650247 5.4.99.1 20 - L-Glu reaction of L-Glu with benzimidazolribofuranosyl-adenosylcobinamide, wild-type enzyme 3458