EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   3.1.3.32 | 0.58 |
- |
dTMP |
- |
652544 |
| 3.1.3.32 | 0.63 |
- |
dTMP |
- |
652544 |
| 3.1.3.32 | 0.833 |
- |
2',3'-cAMP |
mutant enzyme D392E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C |
682186 |
| 3.1.3.32 | 0.92 |
- |
bis-p-nitrophenyl phosphate |
mutant enzyme H264Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2 |
674594 |
| 3.1.3.32 | 0.97 |
- |
2',3'-cAMP |
mutant enzyme H189E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C |
682186 |
| 3.1.3.32 | 1.1 |
- |
2',3'-cAMP |
mutant enzyme D392N, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C |
682186 |
| 3.1.3.32 | 1.1 |
- |
bis-p-nitrophenyl phosphate |
mutant enzyme R237A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2 |
674594 |
| 3.1.3.32 | 1.23 |
- |
bis-p-nitrophenyl phosphate |
mutant enzyme H264A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 |
674594 |
| 3.1.3.32 | 1.45 |
- |
2',3'-cAMP |
mutant enzyme H189A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C |
682186 |
| 3.1.3.32 | 1.67 |
- |
2',3'-cAMP |
mutant enzyme D392A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C |
682186 |
