3.1.3.32 additional information - 2',3'-cAMP mutant enzyme D236E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 0.003 - phosphorylated DNA ends - 652544 3.1.3.32 0.0043 - phosphorylated DNA ends - 652544 3.1.3.32 0.017 - cAMP - 659503 3.1.3.32 0.04 1.97 2',3'-cAMP mutant enzyme R237A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 0.04 1.97 2',3'-cAMP wild type enzyme, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 0.16 - 3'-TMP - 659503 3.1.3.32 0.26 - bis-p-nitrophenyl phosphate mutant enzyme H264Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 0.42 - 3'-phospho-5'-hydroxy-DNA at pH 7.5 and 37°C 730000 3.1.3.32 0.52 - bis-p-nitrophenyl phosphate mutant enzyme D392N, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 0.58 - dTMP - 652544 3.1.3.32 0.63 - dTMP - 652544 3.1.3.32 0.833 - 2',3'-cAMP mutant enzyme D392E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 0.92 - bis-p-nitrophenyl phosphate mutant enzyme H264Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2 674594 3.1.3.32 0.97 - 2',3'-cAMP mutant enzyme H189E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 1.1 - 2',3'-cAMP mutant enzyme D392N, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 1.1 - bis-p-nitrophenyl phosphate mutant enzyme R237A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2 674594 3.1.3.32 1.23 - bis-p-nitrophenyl phosphate mutant enzyme H264A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 1.45 - 2',3'-cAMP mutant enzyme H189A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 1.67 - 2',3'-cAMP mutant enzyme D392A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 1.7 - bis-p-nitrophenyl phosphate wild type enzyme, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2 674594 3.1.3.32 1.88 - p-nitrophenyl phosphate mutant enzyme D236E, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2 674594 3.1.3.32 2.13 - bis-p-nitrophenyl phosphate mutant enzyme H376D, at 45°C, in 50 mM Tris-HCl (pH 8.0) 0.5 mM MnCl2 674594 3.1.3.32 2.5 - p-nitrophenyl phosphate mutant enzyme R237K, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2 674594 3.1.3.32 2.53 - bis-p-nitrophenyl phosphate mutant enzyme D236A, at 45°C, in 50 mM Tris-HCl (pH 8.0) 0.5 mM NiCl2 674594 3.1.3.32 3 6 bis-p-nitrophenyl phosphate mutant enzyme H189D, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 3.13 - bis-p-nitrophenyl phosphate mutant enzyme H376N, at 45°C, in 50 mM Tris-HCl (pH 8.0),0.5 mM MnCl2 674594 3.1.3.32 3.2 - 2',3'-cAMP mutant enzyme H376D, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 3.25 - 2',3'-cAMP mutant enzyme D233E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 3.33 - bis-p-nitrophenyl phosphate mutant enzyme H189Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 3.42 - p-nitrophenyl phosphate mutant enzyme D392E, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2 674594 3.1.3.32 3.5 - 2',3'-cAMP mutant enzyme R237Q, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 3.63 - bis-p-nitrophenyl phosphate mutant enzyme R237K, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2 674594 3.1.3.32 3.72 - bis-p-nitrophenyl phosphate mutant enzyme H189A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 4.05 - bis-p-nitrophenyl phosphate mutant enzyme H376N, at 45°C, in 50 mM Tris-HCl (pH 8.0),0.5 mM MnCl2 674594 3.1.3.32 4.53 - 2',3'-cAMP mutant enzyme D236A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 5.13 - 2',3'-cAMP mutant enzyme H376N, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 5.25 - p-nitrophenyl phosphate mutant enzyme H376N, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2 674594 3.1.3.32 5.77 - 2',3'-cAMP mutant enzyme D236N, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 6.03 - 2',3'-cAMP mutant enzyme D236E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 6.08 - dTMP - 652544 3.1.3.32 6.5 - 2',3'-cAMP mutant enzyme H323Q, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 8.1 - 2',3'-cAMP mutant enzyme R237K, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 8.55 - 2',3'-cAMP mutant enzyme R237A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 8.8 - bis-p-nitrophenyl phosphate mutant enzyme D392E, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 8.93 - 2',3'-cAMP wild type enzyme, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 9.1 - 2',3'-cAMP mutant enzyme H323A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 9.33 - bis-p-nitrophenyl phosphate mutant enzyme D236N, at 45°C, in 50 mM Tris-HCl (pH 8.0) 0.5 mM NiCl2 674594 3.1.3.32 9.58 - p-nitrophenyl phosphate mutant enzyme D236A, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2 674594 3.1.3.32 12 - bis-p-nitrophenyl phosphate mutant enzyme H264N, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2 674594 3.1.3.32 13.77 - bis-p-nitrophenyl phosphate mutant enzyme D392N, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 15 - p-nitrophenyl phosphate wild type enzyme, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2 674594 3.1.3.32 15.97 - 2',3'-cAMP mutant enzyme D392E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 16.9 - bis-p-nitrophenyl phosphate wild type enzyme, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 17.17 - 2',3'-cAMP mutant enzyme H189E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 18 - bis-p-nitrophenyl phosphate mutant enzyme H264A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2 674594 3.1.3.32 18.67 - 2',3'-cAMP mutant enzyme D392N, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 19.33 - bis-p-nitrophenyl phosphate mutant enzyme R237A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2 674594 3.1.3.32 20.17 - 2',3'-cAMP mutant enzyme R237Q, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 23.17 - bis-p-nitrophenyl phosphate wild type enzyme, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 26.17 - 2',3'-cAMP mutant enzyme R237K, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 27.83 - p-Nitrophenyl phenylphosphonate wild type enzyme, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 30.83 - p-nitrophenyl phosphate wild type enzyme, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2 674594 3.1.3.32 33 - bis-p-nitrophenyl phosphate mutant enzyme R237Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2 674594 3.1.3.32 34.33 - 2',3'-cAMP mutant enzyme H189D, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C 682186 3.1.3.32 38.33 - p-nitrophenyl phosphate mutant enzyme D236N, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2 674594 3.1.3.32 43.5 - bis-p-nitrophenyl phosphate mutant enzyme D236A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 52.33 - bis-p-nitrophenyl phosphate mutant enzyme H264Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 65.83 - bis-p-nitrophenyl phosphate mutant enzyme D236N, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 117.5 - bis-p-nitrophenyl phosphate mutant enzyme H189E, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594 3.1.3.32 146.7 - p-nitrophenyl phosphate mutant enzyme H264N, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2 674594 3.1.3.32 253.3 - bis-p-nitrophenyl phosphate mutant enzyme H264A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2 674594