EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
4.2.1.30 | -999 |
- |
- |
33824 |
4.2.1.30 | -999 |
- |
studies on the thermal dissociation of the enzyme |
33820 |
4.2.1.30 | 30 |
- |
30 min, potassium phosphate buffer, pH 8.6, apoenzyme is quite stable. On addition of coenzyme B12 to the incubation mixture the holoenzyme is formed |
33823 |
4.2.1.30 | 45 |
- |
activity is lost dramatically at temperatures above 45°C |
725951 |
4.2.1.30 | 45 |
- |
t1/2: 1 h |
704770 |
4.2.1.30 | 46 |
- |
4 min, 50% destruction of the apoenzyme |
33824 |
4.2.1.30 | 50 |
- |
subunit A: quickly inactivated at temperatures above, K+ and Na+ stabilize at high concentrations |
33820 |
4.2.1.30 | 60 |
- |
dissociation of the inactive enzyme complex with hydroxocobalamin, if the enzyme complex is heated to over 60°C. Heating products: an active subunit B, an inactive subunit A, and a B12 derivative. K+ inhibit heat-mediated dissociation |
33820 |
4.2.1.30 | 70 |
- |
inactivation of subunit A at 70°C is reversible |
33824 |
4.2.1.30 | 70 |
- |
inactive enzyme complex with hydroxocobalamin: quite stable |
33819 |