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Literature summary for 4.2.1.30 extracted from

  • Schneider, Z.; Larsen, E.G.; Jacobson, G.; Johnson, B.C.; Pawelkiewicz, J.
    Purification and properties of glycerol dehydrase (1970), J. Biol. Chem., 245, 3388-3396.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.1 mM AgNO3, apoenzyme: 97% loss of activity, inactive enzyme complex with hydroxocobalamin: 19% loss of activity Klebsiella pneumoniae
EDTA inhibition of the apoenzyme can be completely reversed by Mg2+ in adequate concentrations. Ca2+, Mn2+, Zn2+ and Co2+ also reverse the EDTA inhibitory effect. Glycerol partially reverses the inhibition. Complete inhibition of the enzyme complex with hydroxocobalamin is reversible with an excess of Mg2+ Klebsiella pneumoniae
hexadecyltrimethylammonium bromide 0.14%, complete irreversible inactivation of the apoenzyme Klebsiella pneumoniae
Hg2+ 0.1 mM HgCl2, apoenzyme: complete loss of activity, inactive enzyme complex with hydroxocobalamin: 70% loss of activity Klebsiella pneumoniae
additional information
-
Klebsiella pneumoniae
p-chloromercuribenzoate 0.1 mM, apoenzyme: complete loss of activity, inactive enzyme complex with hydroxocobalamin: 70% loss of activity Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for the reactivation of the active holoenzyme, optimal concentration: 30 mM Klebsiella pneumoniae
SO32- K2SO3, absolute requirement in the reactivation of the inactive enzyme complex with hydroxocobalamin Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
188000
-
sucrose density gradient centrifugation, inactive enzyme complex with hydroxocobalamin Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol Klebsiella pneumoniae
-
?
-
?
glycerol Klebsiella pneumoniae PZH 572 Warsaw
-
?
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae PZH 572 Warsaw
-
-
-

Purification (Commentary)

Purification (Comment) Organism
inactive enzyme complex with hydroxocobalamin Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Klebsiella pneumoniae

Storage Stability

Storage Stability Organism
stable after lyophilization Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol
-
Klebsiella pneumoniae 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
glycerol
-
Klebsiella pneumoniae PZH 572 Warsaw 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
glycerol
-
Klebsiella pneumoniae ?
-
?
glycerol
-
Klebsiella pneumoniae PZH 572 Warsaw ?
-
?

Subunits

Subunits Comment Organism
dimer enzyme dissociates reversibly by action of EDTA into two different very unstable subunits, A and B Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
inactive enzyme complex with hydroxocobalamin: quite stable Klebsiella pneumoniae
78
-
K+ stabilizes inactive enzyme complex with hydroxocobalamin Klebsiella pneumoniae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9.5 inactive enzyme complex with hydroxocobalamin, quite stable between pH 5.0 to pH 9.5, below pH 5.0: inactive enzyme complex with hydroxocobalamin dissociates irreversibly, liberating hydroxocobalamin, B12OH Klebsiella pneumoniae
8 9 apoenzyme is unstable below pH 8.0 and above pH 9.0 Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae
5,6-dimethylbenzimidazolylcobamide 5'-phosphate i.e. DBCC, the inactive enzyme complex with hydroxocobalamin can be converted to the active holoenzyme by replacement of hydroxocobalamin with DBCC in the presence of MG2+ and SO32- Klebsiella pneumoniae