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Literature summary for 4.2.1.30 extracted from
- Molecular cloning, co-expression, and characterization of glycerol dehydratase and 1,3-propanediol dehydrogenase from Citrobacter freundii (2013), Mol. Biotechnol., 54, 469-474.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JM109 cells | Citrobacter freundii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
Propane-1,2-diol | at pH 8.5 and 45°C | Citrobacter freundii |  |
| 0.59 | - |
glycerol | at pH 8.5 and 45°C | Citrobacter freundii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
x * 61000 + x * 22000 + x * 16000, SDS-PAGE | Citrobacter freundii |
| 22000 | - |
x * 61000 + x * 22000 + x * 16000, SDS-PAGE | Citrobacter freundii |
| 61000 | - |
x * 61000 + x * 22000 + x * 16000, SDS-PAGE | Citrobacter freundii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol | Citrobacter freundii | best substrate | 3-hydroxypropanal + H2O | - |
? | |
| glycerol | Citrobacter freundii AS1.1732 | best substrate | 3-hydroxypropanal + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | A8CH84 and A8CH91 and A8CH95 | large, middle and small subunit | - |
| Citrobacter freundii AS1.1732 | A8CH84 and A8CH91 and A8CH95 | large, middle and small subunit | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography and Sephacryl S-300 gel filtration | Citrobacter freundii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.2 | - |
crude extract, at pH 8.5 and 45°C | Citrobacter freundii |
| 91.9 | - |
after 4.3fold purification, at pH 8.5 and 45°C | Citrobacter freundii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol | best substrate | Citrobacter freundii | 3-hydroxypropanal + H2O | - |
? | |
| glycerol | best substrate | Citrobacter freundii AS1.1732 | 3-hydroxypropanal + H2O | - |
? | |
| propane-1,2-diol | - |
Citrobacter freundii | ? | - |
? | |
| propane-1,2-diol | - |
Citrobacter freundii AS1.1732 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61000 + x * 22000 + x * 16000, SDS-PAGE | Citrobacter freundii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DhaB | large subunit | Citrobacter freundii |
| DhaC | middle subunit | Citrobacter freundii |
| DhaE | small subunit | Citrobacter freundii |
| GDHt | - |
Citrobacter freundii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
activity is lost dramatically at temperatures above 45°C | Citrobacter freundii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Citrobacter freundii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | the relative enzyme activity at pH values ranging from 7.0 to 9.5 is over 80%. Activity drops dramatically when the pH value is higher than 9.5 | Citrobacter freundii |
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