EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.5.2.6 | 25 |
- |
60 min, more than 90% of initial activity |
719154 |
| 3.5.2.6 | 25 |
55 |
thermal stability of TEM-149 wild-type and TEM-149T182M mutant is determined by measuring the residual activity after incubation of the enzymes at three different temperatures: 25°C, 40°C, and 55°C. TEM-149 wild-type exhibits half-lives of 90 min at 25°C, 70 min at 40°C, and 30 min at 55°C, whereas TEM-149T182M mutant shows half-lives greater than 120 min at 25°C and 40°C and 45 min at 55°C. Compared to the thermal stability of TEM-149, the thermal stability of the TEM-149T182M mutant is substantially increased |
695638 |
| 3.5.2.6 | 37 |
55 |
residual activity of 82% after 25 min at 37°C and complete loss of activity after 1 min at 60°C or 5 min at 55°C |
690020 |
| 3.5.2.6 | 39.9 |
- |
t1/2 for mutant enzyme W154A, in presence of 1.1 mM sodium bicarbonate |
711200 |
| 3.5.2.6 | 40 |
- |
stable up to |
209323 |
| 3.5.2.6 | 40 |
55 |
the enzyme exhibits a half-life of 90 min at 40°C, 25 min at 45°C, and less than 1 min at 55°C |
684483 |
| 3.5.2.6 | 41.9 |
- |
t1/2 for mutant enzyme W154G, in presence of 1.1 mM sodium bicarbonate |
711200 |
| 3.5.2.6 | 42 |
- |
30 min, mutant enzyme R146S is stable, mutant enzyme K104E loses 50% of maximal activity, mutant enzyme P51L loses 95% of maximal activity, wild-type enzyme loses 80% of maximal activity |
663648 |
| 3.5.2.6 | 44.4 |
- |
and 56.6, transitions in presence of a 10:1 ratio of Zn2+ |
718584 |
| 3.5.2.6 | 48 |
- |
the wild-type Tm is around 48°C occurring over a temperature range of about 23°C (35-58°C) for both the wild-type and the three W229 mutants |
757320 |
