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Literature summary for 3.5.2.6 extracted from

  • Perilli, M.; Caporale, B.; Celenza, G.; Pellegrini, C.; Docquier, J.D.; Mezzatesta, M.; Rossolini, G.M.; Stefani, S.; Amicosante, G.
    Identification and characterization of a new metallo-beta-lactamase, IND-5, from a clinical isolate of Chryseobacterium indologenes (2006), Antimicrob. Agents Chemother., 51, 2988-2990.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Chryseobacterium indologenes

Inhibitors

Inhibitors Comment Organism Structure
1,10-ophenanthroline
-
Chryseobacterium indologenes
dipicolinic acid
-
Chryseobacterium indologenes
EDTA
-
Chryseobacterium indologenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
nitrocefin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
0.013
-
benzylpenicillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
0.025
-
cefazolin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
0.049
-
cefotaxime in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
0.15
-
ampicillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
0.28
-
meropenem in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
0.3
-
Imipenem in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
0.32
-
carbenicillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
0.375
-
piperacillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
1.3
-
Sulbactam in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24540
-
calculated from amino acid sequence Chryseobacterium indologenes
25000
-
SDS-PAGE Chryseobacterium indologenes

Organism

Organism UniProt Comment Textmining
Chryseobacterium indologenes Q6JE29
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono Q Sepharose column chromatography Chryseobacterium indologenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
Chryseobacterium indologenes (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
benzylpenicillin + H2O
-
Chryseobacterium indologenes (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
carbenicillin + H2O
-
Chryseobacterium indologenes (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
cefazolin + H2O
-
Chryseobacterium indologenes (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cefotaxime + H2O
-
Chryseobacterium indologenes (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
imipenem + H2O
-
Chryseobacterium indologenes (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
meropenem + H2O
-
Chryseobacterium indologenes (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
additional information no hydrolysis of ceftazidime, cefepime, moxalactam, tazobactam, and aztreonam Chryseobacterium indologenes ?
-
?
nitrocefin + H2O
-
Chryseobacterium indologenes (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
piperacillin + H2O
-
Chryseobacterium indologenes (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
sulbactam + H2O
-
Chryseobacterium indologenes (2R,4S)-2-(carboxymethyl)-5,5-dimethyl-1,1-dioxo-1lambda~6~,3-thiazolidine-4-carboxylic acid
-
?

Synonyms

Synonyms Comment Organism
IND-5
-
Chryseobacterium indologenes
MBL
-
Chryseobacterium indologenes
metallo-beta-lactamase
-
Chryseobacterium indologenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 55 the enzyme exhibits a half-life of 90 min at 40°C, 25 min at 45°C, and less than 1 min at 55°C Chryseobacterium indologenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.46
-
cefazolin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
1.53
-
cefotaxime in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
3.3
-
Sulbactam in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
4.13
-
meropenem in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
4.4
-
nitrocefin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
7.28
-
Imipenem in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
10.3
-
ampicillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
10.92
-
benzylpenicillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
14.61
-
piperacillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes
16.96
-
carbenicillin in 30 mM sodium cacodylate buffer (pH 6.5) plus 0.05 mM ZnSO4 Chryseobacterium indologenes

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
dipicolinic acid
-
Chryseobacterium indologenes
0.0073
-
1,10-ophenanthroline
-
Chryseobacterium indologenes
0.815
-
EDTA
-
Chryseobacterium indologenes

pI Value

Organism Comment pI Value Maximum pI Value
Chryseobacterium indologenes isoelectric focusing
-
8.8