EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
3.1.3.8 | -999 |
- |
analysis of second virial coefficients of the heavily glycosylated phytase and its enzymatically deglycosylated counterpart. The native forms of phytase and deglycosylated phytase are equally repulsive at the studied pH 8. When thermally denatured, the second virial coefficient of deglycosylated phytase decreases whereas it remaines unchanged for phytase. The expanding effect of the glycans on the denatured protein conformation relies on steric hindrance that limits the range of torsion angles available to the polypeptide |
707750 |
3.1.3.8 | -999 |
- |
better thermostability at 65°C and at 90°C when sodium acetate is used as the buffer, pH 5.5, than that of sodium citrate at either low, 10 mM or high, 0.2 M, concentration |
114250 |
3.1.3.8 | -999 |
- |
immobilized enzyme has higher thermal stability as the untreated enzyme |
114275 |
3.1.3.8 | -999 |
- |
replacement of one alpha-helix on the surface of the Aspergillus terreus phytase by the corresponding stretch of Aspergillus niger phytase results in an enzyme with improved thermostability and unaltered enzymatic activity. The thermostability of this hybrid protein is very similar to that of Aspergillus niger phytase, although the fusion protein contains only a 31 amino acid stretch of the more stable parent enzyme |
652594 |
3.1.3.8 | -999 |
- |
supplemental Ca2+ upgrades both the thermostability and pH stability of recombinant PhyCm |
717000 |
3.1.3.8 | -999 |
- |
temperature profiles of the mutants expressed in Pichia pastoris and Bacillus subtilis |
749620 |
3.1.3.8 | -999 |
- |
the cell-bound phytase exhibits better thermostability than the pure enzyme |
713779 |
3.1.3.8 | -999 |
- |
the temperature stability is increased by Ca2+ |
750984 |
3.1.3.8 | -999 |
- |
thermal stability of the high molecular weight form and the low molecular weight form |
114262 |
3.1.3.8 | 0 |
15 |
purified recombinant His-tagged enzyme, over 50% activity remaining |
751361 |