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Literature summary for 3.1.3.8 extracted from
- Characterization of a thermostable alkaline phytase from Bacillus licheniformis ZJ-6 in Pichia pastoris (2011), World J. Microbiol. Biotechnol., 27, 1247-1253.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene phyC, phylogenetic tree, expression in Pichia pastoris strain GS115 under the control of AOX1 promoter. The recombinant PhyCm, driven by the Saccharomyces cerevisiae alpha-mating factor, is secreted into culture medium | Bacillus licheniformis |
General Stability
| General Stability | Organism |
|---|---|
| supplemental Ca2+ upgrades both the thermostability and pH stability of recombinant PhyCm, overview | Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 25% inhibition at 1 mM, 44% inhibition at 5 mM | Bacillus licheniformis |  |
| Cd2+ | 92% inhibition at 1 mM, 98% inhibition at 5 mM | Bacillus licheniformis | |
| Cu2+ | 32% inhibition at 1 mM, 49% inhibition at 5 mM | Bacillus licheniformis | |
| DTT | 6% inhibition at 5 mM | Bacillus licheniformis | |
| EDTA | 94% inhibition at 1 mM, 100% inhibition at 5 mM | Bacillus licheniformis | |
| Li+ | 10% inhibition at 1 mM, 13% inhibition at 5 mM | Bacillus licheniformis | |
| Mg2+ | 18% inhibition at 1 mM, 27% inhibition at 5 mM | Bacillus licheniformis | |
| Mn2+ | 49% inhibition at 1 mM, 67% inhibition at 5 mM | Bacillus licheniformis | |
| PMSF | 8% inhibition at 1 mM, 10% inhibition at 5 mM | Bacillus licheniformis | |
| Zn2+ | 32% inhibition at 1 mM, 40% inhibition at 5 mM | Bacillus licheniformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stabilizes at 1 mM, activates 20% at 1 mM, 12% at 5 mM | Bacillus licheniformis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | A4UU76 | gene phyC | - |
| Bacillus licheniformis ZJ-6 | A4UU76 | gene phyC | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 3.3fold from culture supernatant of Pichia pastoris strain GS115 by ammonium sulfate fractionation and gel filtration | Bacillus licheniformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.7 | - |
purifed recombinant enzyme, pH 7.5, 60°C | Bacillus licheniformis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| neutral phytase | - |
Bacillus licheniformis |
| PhyCm | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant PhyCm | Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
supplemental Ca2+ upgrades both the thermostability and pH stability of recombinant PhyCm | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
recombinant PhyCm | Bacillus licheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
supplemental Ca2+ upgrades both the thermostability and pH stability of recombinant PhyCm | Bacillus licheniformis |
| 2 | 9 | 37°C, 1 h, over 80% activity remaining of the purified recombinant PhyCm within the range of pH 5.0-9.0, profile, overveiw | Bacillus licheniformis |
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