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Literature summary for 3.1.3.8 extracted from

  • Boukhris, I.; Farhat-Khemakhem, A.; Bouchaala, K.; Virolle, M.J.; Chouayekh, H.
    Cloning and characterization of the first actinomycete beta-propeller phytase from Streptomyces sp. US42 (2016), J. Basic Microbiol., 56, 1080-1089 .
    View publication on PubMed
Search for more literature for 3.1.3.8

Application

Application Comment Organism
agriculture enzyme PHY US42 can be used as feed additive in combination with an acid phytase for monogastric animals Streptomyces sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene phy, DNA and amino acid sequence determination and analysis, sequence comparisons Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
additional information enzyme PHYUS42 exhibits high stability after incubation at 37°C for 1 h in the presence of bovine bile and digestive proteases like pepsin, trypsin, and chymotrypsin. PHY US42 maintains 90% of its activity after incubation for 1 h in the presence of 0.5% bovine bile Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.073
-
 myo-inositol hexakisphosphate pH 7.0, 65°C Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the activity is calcium-dependent, best at 1 mM. Four Ca2+ ions bind to the active Ca2+ binding sites (C3, C5, C6, C7) and create an ideal conformation and charge distribution for the substrate Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol hexakisphosphate + H2O Streptomyces sp.
-
 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O Streptomyces sp. US42
-
 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. A0A0F7VP60 isolated from Tunisian soils
-
Streptomyces sp. US42 A0A0F7VP60 isolated from Tunisian soils
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from cell culture medium 4.2fold by ammonium sulfate frcationation and dialysis Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
 partially purified enzyme, pH 7.0, 65°C Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
 Streptomyces sp. 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Streptomyces sp. US42 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 45795, sequence calcualtion, x * 48000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
beta-propeller phytase
-
 Streptomyces sp.
Phy
-
 Streptomyces sp.
PHY US42
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 80 activity range, profile overview Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the temperature stability is increased by Ca2+ Streptomyces sp.
75
-
 enzyme PHY US42 maintains 80% activity after 10 min in the presence of 5 mM CaCl2, whereas in the absence of calcium, PHY US42 activity is completely abolished after 2 min of incubation Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 myo-inositol hexakisphosphate pH 7.0, 65°C Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9 activity range, inactive below and above, profile overview Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 completely stable at Streptomyces sp.

General Information

General Information Comment Organism
evolution molecular modeling of PHY US42 indicates that this phytase belongs to the group of beta-propeller phytases that are usually calcium-dependent Streptomyces sp.
additional information amino acid sequence analysis and homology structure modeling Streptomyces sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8
-
 myo-inositol hexakisphosphate pH 7.0, 65°C Streptomyces sp.