EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    2.7.7.27 | -999 |
- |
ATP and ADP-Glc, but not Glc-1-P and diphosphate, protect AGPase from thermal inactivation, 3-PGA acts synergistically, overview |
694714 |
| 2.7.7.27 | -999 |
- |
the Arrhenius plots of the data of enzyme assayed in the temperature range 0-40°C exhibit a transition temperature in the range 9-12°C and suggest that the enzyme undergoes conformational changes at different temperatures |
643149 |
| 2.7.7.27 | 0 |
50 |
fully active between 0°C and 50°C for 15 min |
722552 |
| 2.7.7.27 | 25 |
- |
4 days, stable in the presence of 30 mM potassium phosphate, pH 7 |
643133 |
| 2.7.7.27 | 37 |
- |
4 days, stable in the presence of 30 mM potassium phosphate, pH 7 |
643133 |
| 2.7.7.27 | 40 |
- |
the enzyme is unstable at temperatures higher than 40°C in the absence of allosteric activators |
643149 |
| 2.7.7.27 | 42 |
- |
half-lives of wild-type and mutant enzymes lay between 2 and 17 min, overview |
694760 |
| 2.7.7.27 | 42 |
- |
wild-type AGPAse in the absence of 3-phosphoglycerate has a half-life of 1 min, whereas mutant Mos(1-198) has a half-life of 5.5 min, when 3-phosphoglycerate is added to the wid-type enzyme, the half-life increases to 5.5 min and the half-life of the mutant is incrwased to 9.4 min |
694734 |
| 2.7.7.27 | 48 |
- |
half-life: 68 s, irreversible thermal inactivation at 48°C and above, inactivation is temperature- and time-dependendent |
643149 |
| 2.7.7.27 | 50 |
- |
87% remaining activity of the chimeric enzyme AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme, 75% remaining activity of the chimeric enzyme EA contains the N-terminus of Escherichia coli enzyme and the C-terminus of Agrobacterium tumefaciens enzyme and 60% remaining activity of wild-type enzymes |
643152 |
