EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    2.7.4.6 | -999 |
- |
3',5'-cAMP and 3',5'-cGMP at 1 mM stabilize NDPK1, shifting the Tm by 0.9-1.8°C |
761620 |
| 2.7.4.6 | -999 |
- |
mitochondrial form, high thermal stability, Tm 73.5°C |
642671 |
| 2.7.4.6 | -999 |
- |
mutants with a basic amino acid at residue 114 (G114K, G114R) are resistant against heat inactivation as well as against low salt dissociation |
727628 |
| 2.7.4.6 | -999 |
- |
thermal stability of recombinant NDP kinases alpha and beta and their mutant chimeric derivatives, NDP kinase beta, NDP kinase beta1-130alpha131-152, NDP kinase HA-beta1-130alpha131-152, and NDP kinase HA-beta are inactivated at a lower temperature with T1/2 of 51-54°C, overview |
691024 |
| 2.7.4.6 | 20 |
37 |
degeneration during recombinant expression at 37°C, decrease in Kcat/Km with increasing temperature from 20°C to 37°C, Tm is 38°C, inactivation of purified enzyme at 50°C |
739593 |
| 2.7.4.6 | 25 |
- |
t1/2: 30 min |
642655 |
| 2.7.4.6 | 28 |
- |
1 h, 0.0018 mg enzyme/ml, in 0.1 M phosphate buffer, pH 7, more than 50% loss of activity, 2 mg/ml bovine serum albumin plus 2 mM Mg-ADP or 5 mM free ADP protect, not MgCl2 or free ADP alone |
642637 |
| 2.7.4.6 | 30 |
- |
G114S mutant is stable up to 30°C |
727628 |
| 2.7.4.6 | 30 |
80 |
after 5 min incubation, the wild type enzyme loses about 30% activity at 30°C and 100% activity at 40°C in 0.2 M NaCl. In 3.8 M NaCl, the wild type enzyme remains stable up to 75°C and then rapidly loses activity at 80°C |
721435 |
| 2.7.4.6 | 35 |
- |
wild-type enzyme, 0.2 M NaCl, inactivation |
673696 |
