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Literature summary for 2.7.4.6 extracted from

  • Luzarowski, M.; Kosmacz, M.; Sokolowska, E.; Jasinska, W.; Willmitzer, L.; Veyel, D.; Skirycz, A.
    Affinity purification with metabolomic and proteomic analysis unravels diverse roles of nucleoside diphosphate kinases (2017), J. Exp. Bot., 68, 3487-3499 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloned into plant binary vector in which the constitutive 35S promoter is driving the expression of either C- or N-terminally tagged enzyme Arabidopsis thaliana
cloned into plant binary vector in which the constitutive 35S promoter is driving the expression of either C- or N-terminally tagged enzyme (NDPK) Arabidopsis thaliana

General Stability

General Stability Organism
ATP stabilizes the enzyme at a concentration of 0.01 mM Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
cAMP decreases the reaction speed of NDPK1 proportionally to the ligand concentration. Addition of 0.1 mM, 0.2 mM, and 0.5 mM cAMP reduces the specific activity of NDPK1 to 96%, 91%, and 84%, respectively, of the control Arabidopsis thaliana
cGMP decreases the reaction speed of NDPK1 proportionally to the ligand concentration. Addition of 0.1 mM, 0.2 mM, and 0.5 mM cGMP reduces the reaction speed to 84%, 69%, and 51% of the control Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast NDPK2 is exclusively targeted to chloroplasts Arabidopsis thaliana 9507
-
chloroplast NDPK3 is localized to chloroplast Arabidopsis thaliana 9507
-
cytosol predominant localization of NDPK1 in cytosol Arabidopsis thaliana 5829
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O49203
-
-
Arabidopsis thaliana O64903
-
-
Arabidopsis thaliana P39207
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glutathionylation NDPK1 undergoes glutathionylation at Cys43 Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.1
-
pH and temperature not specified in the publication, NDPK1 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + TDP
-
Arabidopsis thaliana ADP + TTP
-
?

Synonyms

Synonyms Comment Organism
At4g09320
-
Arabidopsis thaliana
At4g11010
-
Arabidopsis thaliana
At5g63310
-
Arabidopsis thaliana
NDPK1
-
Arabidopsis thaliana
NDPK2
-
Arabidopsis thaliana
NDPK3
-
Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
3',5'-cAMP and 3',5'-cGMP at 1 mM stabilize NDPK1, shifting the Tm by 0.9-1.8°C Arabidopsis thaliana
60.3
-
Tm-value, nucleoside diphosphate kinase NDPK1, in water Arabidopsis thaliana
60.4
-
Tm-value, nucleoside diphosphate kinase NDPK1, 1 mM IMP, in water Arabidopsis thaliana
60.9
-
Tm-value, nucleoside diphosphate kinase NDPK1, 1 mM adenylosuccinate, in water Arabidopsis thaliana
62.6
-
Tm-value, nucleoside diphosphate kinase NDPK1, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol Arabidopsis thaliana
62.7
-
Tm-value, nucleoside diphosphate kinase NDPK1, 0.01 mM ATP, in water Arabidopsis thaliana
62.7
-
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 0.1 mM cAMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol Arabidopsis thaliana
62.8
-
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 0.1 mM cGMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol Arabidopsis thaliana
63.5
-
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 1 mM cAMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol Arabidopsis thaliana
64.4
-
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 1 mM cGMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function key enzyme regulating NDP/NTP homeostasis Arabidopsis thaliana