Cloned (Comment) | Organism |
---|---|
cloned into plant binary vector in which the constitutive 35S promoter is driving the expression of either C- or N-terminally tagged enzyme | Arabidopsis thaliana |
cloned into plant binary vector in which the constitutive 35S promoter is driving the expression of either C- or N-terminally tagged enzyme (NDPK) | Arabidopsis thaliana |
General Stability | Organism |
---|---|
ATP stabilizes the enzyme at a concentration of 0.01 mM | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cAMP | decreases the reaction speed of NDPK1 proportionally to the ligand concentration. Addition of 0.1 mM, 0.2 mM, and 0.5 mM cAMP reduces the specific activity of NDPK1 to 96%, 91%, and 84%, respectively, of the control | Arabidopsis thaliana | |
cGMP | decreases the reaction speed of NDPK1 proportionally to the ligand concentration. Addition of 0.1 mM, 0.2 mM, and 0.5 mM cGMP reduces the reaction speed to 84%, 69%, and 51% of the control | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | NDPK2 is exclusively targeted to chloroplasts | Arabidopsis thaliana | 9507 | - |
chloroplast | NDPK3 is localized to chloroplast | Arabidopsis thaliana | 9507 | - |
cytosol | predominant localization of NDPK1 in cytosol | Arabidopsis thaliana | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O49203 | - |
- |
Arabidopsis thaliana | O64903 | - |
- |
Arabidopsis thaliana | P39207 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glutathionylation | NDPK1 undergoes glutathionylation at Cys43 | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.1 | - |
pH and temperature not specified in the publication, NDPK1 | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + TDP | - |
Arabidopsis thaliana | ADP + TTP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
At4g09320 | - |
Arabidopsis thaliana |
At4g11010 | - |
Arabidopsis thaliana |
At5g63310 | - |
Arabidopsis thaliana |
NDPK1 | - |
Arabidopsis thaliana |
NDPK2 | - |
Arabidopsis thaliana |
NDPK3 | - |
Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
3',5'-cAMP and 3',5'-cGMP at 1 mM stabilize NDPK1, shifting the Tm by 0.9-1.8°C | Arabidopsis thaliana |
60.3 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, in water | Arabidopsis thaliana |
60.4 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, 1 mM IMP, in water | Arabidopsis thaliana |
60.9 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, 1 mM adenylosuccinate, in water | Arabidopsis thaliana |
62.6 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol | Arabidopsis thaliana |
62.7 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, 0.01 mM ATP, in water | Arabidopsis thaliana |
62.7 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 0.1 mM cAMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol | Arabidopsis thaliana |
62.8 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 0.1 mM cGMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol | Arabidopsis thaliana |
63.5 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 1 mM cAMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol | Arabidopsis thaliana |
64.4 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 1 mM cGMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | key enzyme regulating NDP/NTP homeostasis | Arabidopsis thaliana |