EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    2.7.1.21 | -999 |
- |
2-mercaptoethanol protects against thermal inactivation |
641128 |
| 2.7.1.21 | -999 |
- |
ATP, thymidine, Mg2+ protect against thermal inactivation |
641141 |
| 2.7.1.21 | -999 |
- |
irreversible thermal denaturation following a two-state irrversible denaturation model, study with ligand-bound and free enzyme, respectively, overview |
661195 |
| 2.7.1.21 | 15 |
- |
rLyTK1Met-106: 50% loss of activity within 41 min, rLyTK1Val-106: 50% loss of activity after 392 min |
641158 |
| 2.7.1.21 | 20 |
- |
deletion of the 20 C-terminal amino acids decreases half-life at 20°C from 83 min for wild-type enzyme to 78 min, deletion of the 40 C-terninal amino acids increases half-life to 335 min, deletion of the 44 C-terminal amino acids increases half-life to 500 min |
676243 |
| 2.7.1.21 | 25 |
- |
50% loss of activity within 4 min, rLyTK1Met-106 |
641158 |
| 2.7.1.21 | 25 |
- |
enzyme TK-A and TK-C: half-life 25 h |
641134 |
| 2.7.1.21 | 25 |
40 |
40 min stable |
641139 |
| 2.7.1.21 | 27 |
43 |
stable |
641140 |
| 2.7.1.21 | 37 |
- |
20% loss of activity in the presence of bovine serum albumin, thymidine and dithiothreitol after 60 min, almost complete loss of activity witout bovine serum albumin, thymidine and dithiothreitol after 60 min |
641168 |
