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Literature summary for 2.7.1.21 extracted from

  • Gröbner, P.
    Thymidine kinase enzyme variants in Physarum polycephalum. Kinetics and properties of the enzyme variants (1979), J. Biochem., 86, 1607-1614.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
dGTP
-
Physarum polycephalum
dTDP
-
Physarum polycephalum
dTMP
-
Physarum polycephalum
dTTP
-
Physarum polycephalum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
thymidine TK-A, below 0.004 mM deoxythymidine, 25°C Physarum polycephalum
0.001
-
thymidine TK-C, below 0.004 mM deoxythymidine, 25°C Physarum polycephalum
0.007
-
thymidine TK-A, above 0.004 mM deoxythymidine, 25°C Physarum polycephalum
0.01
-
thymidine TK-C, above 0.004 mM deoxythymidine, 25°C Physarum polycephalum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
sucrose density gradient centrifugation Physarum polycephalum

Organism

Organism UniProt Comment Textmining
Physarum polycephalum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3 variants TK-A, TK-B, TK-C Physarum polycephalum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + thymidine ATP can be substituted by dATP Physarum polycephalum ADP + dTMP
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
enzyme TK-A and TK-C: half-life 25 h Physarum polycephalum
40
-
no activity after 2,5 h Physarum polycephalum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
TK-A and TK-C Physarum polycephalum

pH Range

pH Minimum pH Maximum Comment Organism
6.9 9.2
-
Physarum polycephalum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
dTTP variant TK-A, 25°C Physarum polycephalum
0.0006
-
dTTP variant TK-C, 25°C Physarum polycephalum

pI Value

Organism Comment pI Value Maximum pI Value
Physarum polycephalum variant TK-C 6.8 6.3
Physarum polycephalum variant TK-B
-
7.6
Physarum polycephalum variant TK-A
-
7.8