EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
2.4.1.18 | 35 |
- |
pH 8.0, 30 min, stable below |
636932 |
2.4.1.18 | 51 |
- |
partial inactivation |
636938 |
2.4.1.18 | 70 |
- |
pH 7.0, 30 min, 10% loss of the soluble enzyme form, 50% loss of the insoluble enzyme form |
636942 |
2.4.1.18 | 85 |
- |
soluble and insoluble enzyme form, stable up to |
636942 |
2.4.1.18 | 40 |
- |
pH 8.0, 15 min, stable up to, enzyme form KBE1 |
636945, 636954 |
2.4.1.18 | 50 |
- |
pH 8.0, 15 min, stable up to, enzyme form KBE2 |
636954 |
2.4.1.18 | 50 |
55 |
in the absence of substrates the branching enzyme is stable at 50°C for more than 6 h. At 55°C the half-life of the branching enzyme is 50 min. |
671536 |
2.4.1.18 | -999 |
- |
the activity is lost after incubation at 50°C for 30 min |
674221 |
2.4.1.18 | 90 |
- |
the protein is stable up to 90°C, the activity decreases gradually during the incubation at 100°C |
674295 |
2.4.1.18 | -999 |
- |
when the enzymes are maintained for 15 min at various temperatures ranging from 30°C to 60°C, more than 80% of the original activity was retained up to 47.5°C for PvSBE2, up to 40°C for D15A- and D15E-PvSBE2, up to 42.5°C for R28A- and H24A-PvSBE2, and up to 45°C for R28K-PvSBE2. |
676385 |