EC Number   |
Reference |
|---|
   2.4.1.123 | 7 AtGolS genes, cloning of AtGolS1, 2 and 3, AtGolS1 and 2 are induced by drought and high-salinity stresses, but not by cold stress, AtGolS3 is induced by cold stress, but not by drought or salt stress, overexpression of glutathione S-transferase fusion proteins GTS-AtGolS1, 2 and 3 in Escherichia coli, overexpression of AtGolS2 in transgenic Arabidopsis improves drought tolerance, AtGolS3 is controlled by the transcription factor DREB1A |
638604 |
| 2.4.1.123 | coding region of BhGolS1 is inframe cloned into pGEX-4T-1 downstream to GST coding region and transformed into Escherichia coli BL21(DE3) and BL21 codon plus cells, generation of transgenic tobacco plants, constructs are introduced into Agrobacterium strain LBA4404 by electroporation and transformed into tobacco via a leaf disc method |
706379 |
| 2.4.1.123 | cold-inducible GolS-1 and -2 genes encode 2 distinct galactinol synthases, cloning and sequencing of the GolS-1 and -2 genes, deduced amino acid sequences, expression of GolS-1 cDNA in Escherichia coli as functional enzyme |
638605 |
| 2.4.1.123 | desi and kabuli chickpea genotypes |
736959 |
| 2.4.1.123 | enzyme DNA as cloned into the predigested binary vector, tobacco is transformed by the leaf-disk method using Agrobacterium tumefaciens LBA4404 containing the CsGolS1 recombinant plasmid |
705808 |
| 2.4.1.123 | expression in Escherichia coli |
688075 |
| 2.4.1.123 | expression in Schizosaccharomyces pombe |
685686 |
| 2.4.1.123 | gene GolS1, located on chromosome 3, DNA and amino acid sequence determination and analysis, promoter analysis with isolation, cloning and transient transformation, genetic organization, a non-canonical regulation mechanism controlling the splicing and maturation of rice GolS genes is identified in rice photosynthetic tissue. Two isoforms of Oryza sativa GolS (OsGolS) gene are interspersed by conserved introns harboring characteristic premature termination codons (PTC), recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3) |
760001 |
| 2.4.1.123 | gene GolS2, located on chromosome 7, DNA and amino acid sequence determination and analysis, promoter analysis with isolation, cloning and transient transformation, genetic organization, a non-canonical regulation mechanism controlling the splicing and maturation of rice GolS genes is identified in rice photosynthetic tissue. Two isoforms of Oryza sativa GolS (OsGolS) gene are interspersed by conserved introns harboring characteristic premature termination codons (PTC), recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3) |
760001 |
| 2.4.1.123 | gene MfGolS1, cloned from the cold-treated leaves by reverse transcription PCR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time quantitative PCR enzyme expression analysis, recombinant overexpression in Nicotiana tabacum cv. Zhongyan 90 seeds. Overexpression of MfGolS1 in tobacco results in elevated tolerance to freezing and chilling in transgenic plants as a result of enhanced levels of galactinol, raffinose and stachyose. Tolerance to drought and salt stresses is also increased in the transgenic tobacco plants |
736950 |
