Literature summary for 2.4.1.123 extracted from

Mukherjee, S.; Sengupta, S.; Mukherjee, A.; Basak, P.; Majumder, A.L.
Abiotic stress regulates expression of galactinol synthase genes post-transcriptionally through intron retention in rice (2019), Planta, 249, 891-912 .

Search for more literature for 2.4.1.123

Cloned(Commentary)

Cloned (Comment)	Organism
gene GolS1, located on chromosome 3, DNA and amino acid sequence determination and analysis, promoter analysis with isolation, cloning and transient transformation, genetic organization, a non-canonical regulation mechanism controlling the splicing and maturation of rice GolS genes is identified in rice photosynthetic tissue. Two isoforms of Oryza sativa GolS (OsGolS) gene are interspersed by conserved introns harboring characteristic premature termination codons (PTC), recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)	Oryza sativa Japonica Group
gene GolS2, located on chromosome 7, DNA and amino acid sequence determination and analysis, promoter analysis with isolation, cloning and transient transformation, genetic organization, a non-canonical regulation mechanism controlling the splicing and maturation of rice GolS genes is identified in rice photosynthetic tissue. Two isoforms of Oryza sativa GolS (OsGolS) gene are interspersed by conserved introns harboring characteristic premature termination codons (PTC), recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)	Oryza sativa Japonica Group

Cloned (Comment)

Organism

gene GolS1, located on chromosome 3, DNA and amino acid sequence determination and analysis, promoter analysis with isolation, cloning and transient transformation, genetic organization, a non-canonical regulation mechanism controlling the splicing and maturation of rice GolS genes is identified in rice photosynthetic tissue. Two isoforms of Oryza sativa GolS (OsGolS) gene are interspersed by conserved introns harboring characteristic premature termination codons (PTC), recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)

Oryza sativa Japonica Group

gene GolS2, located on chromosome 7, DNA and amino acid sequence determination and analysis, promoter analysis with isolation, cloning and transient transformation, genetic organization, a non-canonical regulation mechanism controlling the splicing and maturation of rice GolS genes is identified in rice photosynthetic tissue. Two isoforms of Oryza sativa GolS (OsGolS) gene are interspersed by conserved introns harboring characteristic premature termination codons (PTC), recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)

Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-alpha-D-galactose + myo-inositol	Oryza sativa Japonica Group	-	UDP + O-alpha-D-galactosyl-(1->3)-1D-myo-inositol	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa Japonica Group	A0A5S6R7K3	-	-
Oryza sativa Japonica Group	Q40710	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis	Oryza sativa Japonica Group

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	in 15 DAG seedlings, mature full-length OsGolS1 is expressed only in leaf sheath of etiolated plants. In contrast, the q-PCR indicative of pre-mRNA accumulation relative to actin (housekeeping gene) expression shows a strikingly different profile. The pre-mRNA of OsGolS1 is accumulated in the leaf tissue of etiolated plants at a high level, about fourfold to the accumulation of actin. In one-month-old plants, mature OsGolS1 is found only in leaf sheath	Oryza sativa Japonica Group	-
leaf	in one-month-old plants mature OsGolS1 is found only in leaf sheath, OsGolS2 shows both mature and pre-mRNA in sheath. OsGolS2 pre-mRNA is expressed 25fold in leaf sheath	Oryza sativa Japonica Group	-
additional information	plant-wide developmental and tissue-specific profile of isozymes OsGolS1 and OsGolS2 functional expression, overview. In 3-month-old plants, mature OsGolS1 is found in late panicle, shoot, and also in root tissue with a precursor	Oryza sativa Japonica Group	-
additional information	plant-wide developmental and tissue-specific profile of isozymes OsGolS1 and OsGolS2 functional expression, overview. OsGolS2 pre-mRNA is expressed in all light-grown tissue, ranging from 25fold in leaf sheath to twofold in the root compared to actin. The etiolated plants also show high accumulation of pre-mRNA in roots. In one-month-old plants mature OsGolS1 is found only in leaf sheath, OsGolS2 shows both mature and pre-mRNA in sheath. OsGolS2 is not found in 3-month-old plants	Oryza sativa Japonica Group	-
panicle	-	Oryza sativa Japonica Group	-
root	-	Oryza sativa Japonica Group	-
seedling	-	Oryza sativa Japonica Group	-
shoot	-	Oryza sativa Japonica Group	-
spikelet	-	Oryza sativa Japonica Group	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-alpha-D-galactose + myo-inositol	-	Oryza sativa Japonica Group	UDP + O-alpha-D-galactosyl-(1->3)-1D-myo-inositol	-	?

Synonyms

Synonyms	Comment	Organism
galactinol synthase	-	Oryza sativa Japonica Group
OJ1165_F02.103	locus name	Oryza sativa Japonica Group
Os07g0687900	locus name	Oryza sativa Japonica Group
OsGolS1	-	Oryza sativa Japonica Group
OsGolS2	-	Oryza sativa Japonica Group

Expression

Organism	Comment	Expression
Oryza sativa Japonica Group	expression of GolS genes is implicated in abiotic stress, especially drought and salinity, overview. A non-canonical regulation mechanism controlling the splicing and maturation of rice GolS genes is identified in rice photosynthetic tissue. Two isoforms of Oryza sativa GolS (OsGolS) gene, located in chromosomes 3(OsGolS1) and 7(OsGolS2), are interspersed by conserved introns harboring characteristic premature termination codons (PTC). During abiotic stress, the premature and mature transcripts of both isoforms accumulate in a rhythmic manner for very small time-windows interrupted by phases of complete absence. Reporter gene assay using GolS promoters under abiotic stress does not reflect this accumulation profile, suggesting that this regulation occurs post-transcriptionally. Analysis reveals that a combined transcriptional and post transcriptional control exists in rice to regulate GolS expression under stress. Selective degradation of OsGolS pre-mRNAs	additional information

General Information

General Information	Comment	Organism
metabolism	the enzyme catalyzes the first step of galactinol biosynthesis, overview. Galactinol and its adducts of the raffinose family oligosaccharides (RFO) series are implicated in carbon storage, transport, maintaining the source-sink relationship, stress amelioration, and signalling	Oryza sativa Japonica Group