EC Number   |
Reference |
|---|
   4.2.2.3 | recombinant expression of N-terminally GST-tagged enzyme in Escherichia coli strains BL21(DE3), C41(DE3) and C43(DE3) |
729534 |
| 4.2.2.3 | sequence comparison of PL-5 alginate lyase, recombinant wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS |
728866 |
| 4.2.2.3 | subcloning of His-tagged wild-type and mutant enzymes in Escherichia coli strain DH5-alpha, expression in Escherichia coli strain BL21(DE3) Rosetta, the enzymes are secreted from periplasmic space |
730063 |
| 4.2.2.3 | the 3 isozymes are natively expressed as 1 precursor molecule and are posttranslationally processed to monomeric forms, overexpression of all isozymes A1-I, A1-II, and A1-III in Escherichia coli |
653728 |
| 4.2.2.3 | when the alginate lysase gene (aly) is expressed in Escherichi coli, most of the gene product is organized as aggregated insoluble particles. At least 37% of the total alginate lyase is produced in the soluble fraction when aly is coexpressed with DnaK/DnaJ/GrpE chaperone |
678853 |
