4.2.2.3 - 34077, 648532, 747722 4.2.2.3 AkAly30 DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) 714373 4.2.2.3 AlgL is co-expressed in the algD operon 694031 4.2.2.3 algL is expressed in pET24a (+)/E. coli BL21 (DE3) system 682995 4.2.2.3 baculoviral expression system 705483 4.2.2.3 DNA and amino acid sequence determination and analysis 653039 4.2.2.3 DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 729058 4.2.2.3 DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) at 37°C and at 15°C. The recombinant alginate lyase expressed with pColdI at 15°C exhibits the highest alginate-degrading activity, method evaluation and optimization 714484 4.2.2.3 DNA and amino acid sequence determination and analysis, lambda phage display, sequence comparisons 730133 4.2.2.3 DNA and amino acid sequence determination and analysis, phylogenetic analysis 729531 4.2.2.3 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 716091 4.2.2.3 DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 729535 4.2.2.3 DNA sequence determination and analysis of the gene encoding isozyme A1-III, expression of wild-type and mutant H192A in Escherichia coli 652765 4.2.2.3 expressed as a His-tagged fusion protein 693648 4.2.2.3 expressed as a His-tagged fusion protein in Escherichia coli 693967 4.2.2.3 expressed as secreted proteins with baculoviral expression systems in Sf9 cells 705483 4.2.2.3 expressed in Escherichia coli B834(DE3) cells 704682 4.2.2.3 expressed in Escherichia coli BL21(DE3) cells 702993 4.2.2.3 expressed in Escherichia coli DH5alpha cells 704300, 705481 4.2.2.3 expressed in yeast and Escherichia coli as a His-tagged fusion protein 692125 4.2.2.3 expression in Escherichia coli 34065, 34076, 664733, 679049, 704682, 705481, 716091, 746826, 747844, 747854, 747945, 747949, 748129, 748210, 748341, 749227, 749289 4.2.2.3 expression in Escherichia coli. Various calcium compounds that enhance the expression of the active enzyme. Maximum activity is observed in the clones cultured in broth containing 50-100% artificial seawater, respectively 679563 4.2.2.3 expression in Escheriochia coli 747942 4.2.2.3 expression in T7 driven pET vector system 716698 4.2.2.3 expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli 716698 4.2.2.3 expression of C-terminally His6-tagged A1-III 728975 4.2.2.3 expression of C-terminally His6-tagged AlgL 728975 4.2.2.3 expression of native A1-II' and a truncated A1-II' lacking the N-terminal 80 amino acid residues in Escherichia coli 665371 4.2.2.3 gene alg17C, expression in Escherichia coli strain BL21(DE3) 729056 4.2.2.3 gene alg7D, overexpression of soluble His-tagged enzyme lacking the signal peptide in Escherichia coli strain BL21(DE3) 729459 4.2.2.3 gene algE1-1 652156 4.2.2.3 gene algL, algL is part of the alginate biosynthetic operon, expression of His-tagged enzyme in Escherichia coli strain C41(DE3) 729210 4.2.2.3 gene algL, construction of a mutant strain SML2, by introductionof a non-polar mutation, with no alginate lyase activity 649405 4.2.2.3 gene algL, DNA and amino acid sequence determination and analysis, cloning and overexpression in Escherichia coli 651639 4.2.2.3 gene algL, DNA sequence determination and analysis, overexpression in Escherichia coli BL21(DE3), periplasmic enzyme location 651668 4.2.2.3 gene algL, expression from pNMAlgL28–362 expression vector as His-tagged enzyme in Escherichia coli strain Origami 2 (DE3) 728899 4.2.2.3 gene alyA, expression in Escherichia coli strain DH5alpha 715553 4.2.2.3 gene alyVI, expression of GST-fusion wild-type and mutant enzymes in Escherichia coli strain BL-21 LysS 729276 4.2.2.3 gene cl2, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein 651347 4.2.2.3 gene encoding the oligoalginate lyase, DNA sequence determination and analysis, genetic mapping 651661 4.2.2.3 gene of alginate lyase (AlyVI) from marine bacterium Vibrio is cloned into the multiple cloning site of the surface display vector pINA1317-YlCWP110 and expressed in cells of Yarrowia lipolytica 693970 4.2.2.3 mutant enzymes H202Q and Y256F 665388 4.2.2.3 overexpressed in Escherichia coli 682820, 695268 4.2.2.3 overexpression in Escherichia coli 665769 4.2.2.3 overexpression of AlgL in Escherichia coli 665543 4.2.2.3 overexpression of isozyme A1-II in Escherichia coli 650305 4.2.2.3 overexpression of isozyme A1-III in Bacillus subtilis 652853 4.2.2.3 phylogenetic analysis 715322 4.2.2.3 phylogenetic analysis, recombinant expression of His-tagged AlyA5 in Escherichia coli strain BL21(DE3) 730018 4.2.2.3 protein PA1167, overexpression in Escherichia coli 665543 4.2.2.3 recombinant expression of N-terminally GST-tagged enzyme in Escherichia coli strains BL21(DE3), C41(DE3) and C43(DE3) 729534 4.2.2.3 sequence comparison of PL-5 alginate lyase, recombinant wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS 728866 4.2.2.3 subcloning of His-tagged wild-type and mutant enzymes in Escherichia coli strain DH5-alpha, expression in Escherichia coli strain BL21(DE3) Rosetta, the enzymes are secreted from periplasmic space 730063 4.2.2.3 the 3 isozymes are natively expressed as 1 precursor molecule and are posttranslationally processed to monomeric forms, overexpression of all isozymes A1-I, A1-II, and A1-III in Escherichia coli 653728 4.2.2.3 when the alginate lysase gene (aly) is expressed in Escherichi coli, most of the gene product is organized as aggregated insoluble particles. At least 37% of the total alginate lyase is produced in the soluble fraction when aly is coexpressed with DnaK/DnaJ/GrpE chaperone 678853