Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | isozymes A1-I and A1-II are not affected by addition of 1 mM EDTA, DTT, GSH, 2-mercaptoethanol, iodoacetic acid, and sugars, L-fucose, D-galactose, D-glucose, D-glucuronic acid, D-mannose, L-rhamnose, and D-xylose, no effect on isozymes A1-I and A1-II of Ca2+, Co2+, Mg2+, and Mn2+ at 1 mM | Sphingomonas sp. |
Cloned (Comment) | Organism |
---|---|
the 3 isozymes are natively expressed as 1 precursor molecule and are posttranslationally processed to monomeric forms, overexpression of all isozymes A1-I, A1-II, and A1-III in Escherichia coli | Sphingomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 40% inhibition at 1 mM of isozyme A1-I and A1-II | Sphingomonas sp. | |
additional information | isozymes A1-I and A1-II are not affected by addition of 1 mM EDTA, DTT, GSH, 2-mercaptoethanol, iodoacetic acid, and sugars, L-fucose, D-galactose, D-glucose, D-glucuronic acid, D-mannose, L-rhamnose, and D-xylose, no effect on isozymes A1-I and A1-II of Ca2+, Co2+, Mg2+, and Mn2+ at 1 mM | Sphingomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
isozyme A1-II, gel filtration | Sphingomonas sp. |
40000 | - |
isozyme A1-III, gel filtration | Sphingomonas sp. |
63000 | - |
1 * 63000, A1-I, SDS-PAGE, 1 * 25000, A1-II, SDS-PAGE, 1 * 40000, A1-III, SDS-PAGE | Sphingomonas sp. |
66000 | - |
isozyme A1-I, gel filtration | Sphingomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas sp. | - |
isoforms A1-I, A1-II, and A1-III | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | A1-I is autolytically cleaved into A1-II and A1-III, which shows different substrate specificities, therfore A1-I possesses 3 active sites, 1 protease and 2 alginate lyase sites | Sphingomonas sp. |
Purification (Comment) | Organism |
---|---|
recombinant isozymes A1-I 11.2fold, A1-II 25.5fold, and A1-III 4.9fold, from Escherichia coli | Sphingomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
45 | - |
purified isozyme A1-III | Sphingomonas sp. |
73.1 | - |
purified isozyme A1-I | Sphingomonas sp. |
109 | - |
purified isozyme A1-II | Sphingomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | isozyme A1-II and A1-I: endolytic cleavage of the glycosidic bonds, beta-elimination reaction, A1-II shows a preference for polyguluronate instead of polymannuronate | Sphingomonas sp. | unsaturated algino-oligosaccharides | A1-I produces mainly di- and trisaccharides, A1-II produces mainly tri- and tetrasaccharides | ? | |
additional information | no activity with gellan, xanthan, and pectin of A1-I and A1-II | Sphingomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 63000, A1-I, SDS-PAGE, 1 * 25000, A1-II, SDS-PAGE, 1 * 40000, A1-III, SDS-PAGE | Sphingomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
alginate lyase | - |
Sphingomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sphingomonas sp. |
45 | - |
isozyme A1-I | Sphingomonas sp. |
70 | - |
isozyme A1-I | Sphingomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
pH 7.2, 10 min, loss f 50% activity, isozyme A1-I | Sphingomonas sp. |
50 | - |
pH 7.2, 10 min, loss f 50% activity, isozyme A1-I | Sphingomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Sphingomonas sp. |
8 | - |
isozymes A1-I and A1-II | Sphingomonas sp. |