BRENDA - Enzyme Database
show all sequences of 4.2.2.3

Overexpression in Escherichia coli, purification, and characterization of Sphingomonas sp. A1 alginate lyases

Yoon, H.J.; Hashimoto, W.; Miyake, O.; Okamoto, M.; Mikami, B.; Murata, K.; Protein Expr. Purif. 19, 84-90 (2000)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
isozymes A1-I and A1-II are not affected by addition of 1 mM EDTA, DTT, GSH, 2-mercaptoethanol, iodoacetic acid, and sugars, L-fucose, D-galactose, D-glucose, D-glucuronic acid, D-mannose, L-rhamnose, and D-xylose, no effect on isozymes A1-I and A1-II of Ca2+, Co2+, Mg2+, and Mn2+ at 1 mM
Sphingomonas sp.
Cloned(Commentary)
Commentary
Organism
the 3 isozymes are natively expressed as 1 precursor molecule and are posttranslationally processed to monomeric forms, overexpression of all isozymes A1-I, A1-II, and A1-III in Escherichia coli
Sphingomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
Hg2+
40% inhibition at 1 mM of isozyme A1-I and A1-II
Sphingomonas sp.
additional information
isozymes A1-I and A1-II are not affected by addition of 1 mM EDTA, DTT, GSH, 2-mercaptoethanol, iodoacetic acid, and sugars, L-fucose, D-galactose, D-glucose, D-glucuronic acid, D-mannose, L-rhamnose, and D-xylose, no effect on isozymes A1-I and A1-II of Ca2+, Co2+, Mg2+, and Mn2+ at 1 mM
Sphingomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
isozyme A1-II, gel filtration
Sphingomonas sp.
40000
-
isozyme A1-III, gel filtration
Sphingomonas sp.
63000
-
1 * 63000, A1-I, SDS-PAGE, 1 * 25000, A1-II, SDS-PAGE, 1 * 40000, A1-III, SDS-PAGE
Sphingomonas sp.
66000
-
isozyme A1-I, gel filtration
Sphingomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sphingomonas sp.
-
isoforms A1-I, A1-II, and A1-III
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
A1-I is autolytically cleaved into A1-II and A1-III, which shows different substrate specificities, therfore A1-I possesses 3 active sites, 1 protease and 2 alginate lyase sites
Sphingomonas sp.
Purification (Commentary)
Commentary
Organism
recombinant isozymes A1-I 11.2fold, A1-II 25.5fold, and A1-III 4.9fold, from Escherichia coli
Sphingomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
45
-
purified isozyme A1-III
Sphingomonas sp.
73.1
-
purified isozyme A1-I
Sphingomonas sp.
109
-
purified isozyme A1-II
Sphingomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alginate
isozyme A1-II and A1-I: endolytic cleavage of the glycosidic bonds, beta-elimination reaction, A1-II shows a preference for polyguluronate instead of polymannuronate
653728
Sphingomonas sp.
oligosaccharides
A1-I produces mainly di- and trisaccharides, A1-II produces mainly tri- and tetrasaccharides
653728
Sphingomonas sp.
?
additional information
no activity with gellan, xanthan, and pectin of A1-I and A1-II
653728
Sphingomonas sp.
?
-
653728
Sphingomonas sp.
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 63000, A1-I, SDS-PAGE, 1 * 25000, A1-II, SDS-PAGE, 1 * 40000, A1-III, SDS-PAGE
Sphingomonas sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Sphingomonas sp.
45
-
isozyme A1-I
Sphingomonas sp.
70
-
isozyme A1-I
Sphingomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
pH 7.2, 10 min, loss f 50% activity, isozyme A1-I
Sphingomonas sp.
50
-
pH 7.2, 10 min, loss f 50% activity, isozyme A1-I
Sphingomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Sphingomonas sp.
8
-
isozymes A1-I and A1-II
Sphingomonas sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
isozymes A1-I and A1-II are not affected by addition of 1 mM EDTA, DTT, GSH, 2-mercaptoethanol, iodoacetic acid, and sugars, L-fucose, D-galactose, D-glucose, D-glucuronic acid, D-mannose, L-rhamnose, and D-xylose, no effect on isozymes A1-I and A1-II of Ca2+, Co2+, Mg2+, and Mn2+ at 1 mM
Sphingomonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
the 3 isozymes are natively expressed as 1 precursor molecule and are posttranslationally processed to monomeric forms, overexpression of all isozymes A1-I, A1-II, and A1-III in Escherichia coli
Sphingomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hg2+
40% inhibition at 1 mM of isozyme A1-I and A1-II
Sphingomonas sp.
additional information
isozymes A1-I and A1-II are not affected by addition of 1 mM EDTA, DTT, GSH, 2-mercaptoethanol, iodoacetic acid, and sugars, L-fucose, D-galactose, D-glucose, D-glucuronic acid, D-mannose, L-rhamnose, and D-xylose, no effect on isozymes A1-I and A1-II of Ca2+, Co2+, Mg2+, and Mn2+ at 1 mM
Sphingomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
isozyme A1-II, gel filtration
Sphingomonas sp.
40000
-
isozyme A1-III, gel filtration
Sphingomonas sp.
63000
-
1 * 63000, A1-I, SDS-PAGE, 1 * 25000, A1-II, SDS-PAGE, 1 * 40000, A1-III, SDS-PAGE
Sphingomonas sp.
66000
-
isozyme A1-I, gel filtration
Sphingomonas sp.
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
A1-I is autolytically cleaved into A1-II and A1-III, which shows different substrate specificities, therfore A1-I possesses 3 active sites, 1 protease and 2 alginate lyase sites
Sphingomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant isozymes A1-I 11.2fold, A1-II 25.5fold, and A1-III 4.9fold, from Escherichia coli
Sphingomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
45
-
purified isozyme A1-III
Sphingomonas sp.
73.1
-
purified isozyme A1-I
Sphingomonas sp.
109
-
purified isozyme A1-II
Sphingomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alginate
isozyme A1-II and A1-I: endolytic cleavage of the glycosidic bonds, beta-elimination reaction, A1-II shows a preference for polyguluronate instead of polymannuronate
653728
Sphingomonas sp.
oligosaccharides
A1-I produces mainly di- and trisaccharides, A1-II produces mainly tri- and tetrasaccharides
653728
Sphingomonas sp.
?
additional information
no activity with gellan, xanthan, and pectin of A1-I and A1-II
653728
Sphingomonas sp.
?
-
653728
Sphingomonas sp.
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 63000, A1-I, SDS-PAGE, 1 * 25000, A1-II, SDS-PAGE, 1 * 40000, A1-III, SDS-PAGE
Sphingomonas sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Sphingomonas sp.
45
-
isozyme A1-I
Sphingomonas sp.
70
-
isozyme A1-I
Sphingomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
pH 7.2, 10 min, loss f 50% activity, isozyme A1-I
Sphingomonas sp.
50
-
pH 7.2, 10 min, loss f 50% activity, isozyme A1-I
Sphingomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Sphingomonas sp.
8
-
isozymes A1-I and A1-II
Sphingomonas sp.
Other publictions for EC 4.2.2.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
16
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1
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6
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1
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3
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3
1
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1
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3
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3
1
1
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3
1
1
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1
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2
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3
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3
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3
1
1
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3
1
1
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-
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3
3
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163
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-
1
-
-
-
11
-
-
-
-
-
-
5
-
-
1
-
1
-
-
-
5
1
1
-
-
-
1
1
-
-
-
1
-
-
-
1
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
1
1
-
-
-
5
1
1
-
-
-
1
1
-
1
-
-
-
-
-
-
748341
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37
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-
1
-
-
-
-
1
-
-
-
-
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1
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-
-
-
-
1
1
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3
1
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1
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1
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1
1
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3
1
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-
-
-
-
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-
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16
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-
-
-
-
-
5
3
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3
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4
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3
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6
1
1
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1
3
1
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1
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5
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3
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3
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3
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6
1
1
-
1
3
1
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1
-
-
-
-
-
3
3
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2
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-
-
-
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2
-
-
-
-
2
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-
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1
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-
-
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-
-
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2
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4
1
1
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1
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1
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1
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1
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1
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4
1
1
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1
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1
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1
1
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1
1
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3
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3
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1
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3
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1
1
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1
3
1
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1
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1
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3
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4
1
1
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1
3
1
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1
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3
3
747995
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21
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2017
-
-
-
-
-
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-
-
-
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7
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2
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1
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1
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2
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1
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1
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1
1
1
1
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748221
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Aplysia kurodai
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292
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2017
-
-
-
1
4
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
14
-
4
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
1
4
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3
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14
-
4
-
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-
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749331
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7
13656
2017
-
-
-
-
-
-
1
3
-
-
-
-
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3
-
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3
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1
1
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3
1
1
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1
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3
-
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-
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-
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3
-
1
1
-
3
1
1
-
-
-
1
1
-
3
3
747319
Yagi
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Biosci. Biotechnol. Biochem.
80
2338-2346
2016
-
-
-
-
-
-
1
-
1
2
-
-
-
5
-
1
1
-
-
-
-
-
4
1
1
-
2
-
1
1
-
-
-
1
-
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-
-
-
-
-
-
-
1
-
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1
2
-
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-
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1
1
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-
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4
1
1
-
2
-
1
1
-
1
-
-
-
-
-
-
747844
Chen
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Front. Microbiol.
7
1120
2016
-
-
1
-
-
-
1
-
-
6
-
-
-
3
-
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-
-
-
-
-
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3
1
1
1
2
-
1
1
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1
-
-
-
-
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1
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6
-
-
-
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-
-
-
-
-
-
3
1
1
1
2
-
1
1
-
-
-
-
-
-
-
-
748210
Inoue
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291
15551-15563
2016
-
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1
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Characterization of a new end ...
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Purification and characterizat ...
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Purification and characterisat ...
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Cloning and characterization o ...
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Wolfram
Expression, purification, crys ...
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Kim
Depolymerization of alginate i ...
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Lee
Molecular cloning, purificatio ...
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Farrell
Functional characterization of ...
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Biochemistry
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Rahman
cDNA cloning and bacterial exp ...
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Characterization of alginate l ...
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Protoplast preparation from La ...
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Li
Three alginate lyases from mar ...
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Rahman
cDNA cloning of an alginate ly ...
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Biochimie
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Heterologous expression of an ...
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Li
Preparation, purification and ...
Pseudomonas sp., Pseudomonas sp. HZJ 216
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2011
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Singh
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Aspergillus oryzae
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Li
Purification and characterizat ...
Pseudoalteromonas sp.
Mar. Drugs
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716698
Lamppa
Genetically engineered alginat ...
Sphingomonas sp.
PLoS ONE
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2011
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729276
Hamza
Insight into the binding of th ...
Vibrio sp., Vibrio sp. QY105
Biochim. Biophys. Acta
1814
1739-1747
2011
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701884
Matsushima
Analysis of extracellular algi ...
Pseudoalteromonas atlantica, Pseudoalteromonas atlantica AR06
Appl. Microbiol. Biotechnol.
86
567-576
2010
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4
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1
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1
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1
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Ochiai
Crystal structure of exotype a ...
Agrobacterium tumefaciens
J. Biol. Chem.
285
24519-24528
2010
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2
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705481
Uchimura
Cloning and sequencing of algi ...
Agarivorans sp., Agarivorans sp. JAM-A1m, Vibrio sp., Vibrio sp. A9m
Mar. Biotechnol.
12
526-533
2010
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2
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8
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14
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4
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7
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7
8
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4
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1
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16
2
1
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1
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2
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4
4
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714770
Rahman
Isolation and characterization ...
Aplysia kurodai
Comp. Biochem. Physiol. B
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317-325
2010
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3
3
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5
2
1
1
1
-
1
1
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1
1
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715553
Tondervik
Isolation of mutant alginate l ...
Klebsiella pneumoniae, Klebsiella pneumoniae SM0524
J. Biol. Chem.
285
35284-35292
2010
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21
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1
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1
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4
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1
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1
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692811
An
Alginate-deriving oligosacchar ...
Flavobacterium sp.
J. Appl. Microbiol.
106
161-170
2009
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1
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1
1
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1
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693942
Tang
Isolation and characterization ...
Gracilibacillus sp. A7
Lett. Appl. Microbiol.
48
38-43
2009
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1
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693967
Kim
Cloning and Characterization o ...
Streptomyces sp.
Mar. Biotechnol.
11
10-16
2009
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1
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1
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1
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1
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1
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1
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693970
Liu
The Surface Display of the Alg ...
Vibrio sp.
Mar. Biotechnol.
11
619-626
2009
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1
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3
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1
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3
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1
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2
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701807
Hay
Impact of alginate overproduct ...
Flavobacterium sp.
Appl. Environ. Microbiol.
75
6022-6025
2009
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1
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702993
Duan
Cloning, sequence analysis, an ...
Pseudoalteromonas sp.
Can. J. Microbiol.
55
1113-1118
2009
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1
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9
1
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5
2
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4
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1
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1
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1
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1
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1
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9
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2
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1
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1
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1
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1
1
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703603
Kobayashi
A new high-alkaline alginate l ...
Agarivorans sp., Agarivorans sp. JAM-A1m
Extremophiles
13
121-129
2009
9
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10
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2
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1
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9
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10
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2
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1
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2
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2
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1
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1
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1
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1
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703781
Hata
-
Comparative study on general p ...
Haliotis discus hannai, Haliotis iris, Littorina brevicula, Omphalius rusticus
Fish. Sci.
75
755-763
2009
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6
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4
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6
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4
-
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4
-
4
-
4
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4
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4
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704225
Alipour
Importance of DNase and algina ...
Homo sapiens
J. Antimicrob. Chemother.
64
317-325
2009
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1
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704300
Gimmestad
Characterization of three new ...
Azotobacter vinelandii
J. Bacteriol.
191
4845-4853
2009
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1
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3
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3
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1
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4
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-
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3
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3
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1
1
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705439
Lee
-
Identification and characteriz ...
Pseudomonas sp., Pseudomonas sp. N7151-6
Korean J. Microbiol. Biotechnol.
37
350-354
2009
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1
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1
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1
1
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1
1
-
-
-
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692062
Volpi
Micellar electrokinetic capill ...
Flavobacterium sp.
Electrophoresis
29
3504-3510
2008
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1
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1
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1
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692125
Yamamoto
-
Catalytically important amino- ...
Haliotis discus hannai
Enzyme Microb. Technol.
43
396-402
2008
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1
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6
-
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1
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1
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1
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2
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2
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1
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6
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1
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1
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2
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2
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693648
Ogura
Substrate recognition by famil ...
Sphingomonas sp.
J. Mol. Biol.
380
373-385
2008
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1
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3
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2
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1
1
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1
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1
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1
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3
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1
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1
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1
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1
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1
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694031
Oglesby
Membrane topology and roles of ...
Pseudomonas aeruginosa
Microbiology
154
1605-1615
2008
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1
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1
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-
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-
-
695268
Ma
-
Overexpression of alginate lya ...
Pseudoalteromonas elyakovii
World J. Microbiol. Biotechnol.
24
89-96
2008
6
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1
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-
-
8
-
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6
1
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1
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1
-
-
-
-
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3
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1
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1
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2
1
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6
-
1
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8
-
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6
1
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1
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3
-
1
-
1
-
2
1
-
-
-
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-
-
-
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678566
Doumeche
Enzyme-catalyzed phase transit ...
Flavobacterium sp.
Biomacromolecules
8
3613-3618
2007
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1
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678571
Ashton
Scaffolds based on degradable ...
Homo sapiens
Biomaterials
28
5518-5525
2007
-
1
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2
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678903
Breguet
Characterization of alginate l ...
Flavobacterium sp.
Biotechnol. Prog.
23
1223-1230
2007
-
1
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1
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1
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1
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1
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1
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1
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1
-
1
-
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-
1
-
-
-
-
-
-
-
-
-
679563
Sawabe
-
Enhanced expression of active ...
Pseudoalteromonas elyakovii
Enzyme Microb. Technol.
40
285-291
2007
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1
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1
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-
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-
-
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680308
Cao
Purification and characterizat ...
Streptomyces sp., Streptomyces sp. A5
J. Agric. Food Chem.
55
5113-5117
2007
1
1
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1
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1
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4
1
1
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1
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1
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1
1
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4
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1
1
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1
2
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1
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4
1
1
-
1
-
1
-
-
-
-
-
-
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-
-
663672
Alkawash
Alginate lyase enhances antibi ...
Pseudomonas aeruginosa
Apmis
114
131-138
2006
-
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-
-
-
-
-
-
-
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-
-
-
-
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677288
Wang
Purification and characterizat ...
Vibrio sp., Vibrio sp. YWA
Acta Biochim. Biophys. Sin.
38
633-638
2006
1
-
-
-
-
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1
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1
1
1
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4
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1
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-
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-
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2
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1
1
4
-
1
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1
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1
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1
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1
1
1
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1
-
-
-
-
2
-
1
1
4
-
1
-
1
-
-
-
-
-
-
-
677348
Ochiai
Crystallization and preliminar ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
Acta Crystallogr. Sect. F
62
486-488
2006
-
-
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1
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22
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1
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-
-
-
-
-
-
-
-
-
-
678853
Shin
-
Effect of molecular chaperones ...
Pseudoalteromonas elyakovii
Biotechnol. Bioprocess Eng.
11
414-419
2006
-
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1
-
-
-
-
-
-
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1
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679049
Suzuki
A novel oligoalginate lyase fr ...
Haliotis discus hannai
Carbohydr. Res.
341
1809-1819
2006
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1
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1
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4
2
2
1
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2
1
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1
1
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-
679376
Hu
Purification and characterizat ...
Vibrio sp.
Curr. Microbiol.
53
135-140
2006
-
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1
5
1
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4
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1
1
1
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1
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1
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1
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-
679551
Shen
-
A high efficient electroporati ...
Vibrio sp.
Enzyme Microb. Technol.
39
677-682
2006
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1
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682820
Ochiai
A biosystem for alginate metab ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
Res. Microbiol.
157
642-649
2006
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4
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2
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26
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4
1
1
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1
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-
682995
Xiao
-
A novel alginate lyase with hi ...
Pseudomonas sp., Pseudomonas sp. QD03
World J. Microbiol. Biotechnol.
22
81-88
2006
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1
1
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5
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1
5
2
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2
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5
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1
1
1
1
5
-
1
1
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-
663527
Yamasaki
Crystallization and preliminar ...
Sphingomonas sp.
Acta Crystallogr. Sect. F
61
288-290
2005
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1
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665176
Jain
Role of an alginate lyase for ...
Pseudomonas aeruginosa
Infect. Immun.
73
6429-6436
2005
-
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5
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2
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665388
Albrecht
Alginate lyase (AlgL) activity ...
Pseudomonas aeruginosa
J. Bacteriol.
187
3869-3872
2005
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1
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2
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1
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665399
Bakkevig
Role of the Pseudomonas fluore ...
Pseudomonas fluorescens
J. Bacteriol.
187
8375-8384
2005
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1
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-
665769
Hashimoto
Molecular identification of Sp ...
Sphingomonas sp.
J. Biosci. Bioeng.
99
48-54
2005
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1
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4
1
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1
1
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5
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1
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1
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4
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1
1
1
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1
1
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4
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1
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1
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1
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1
-
4
-
1
1
1
-
1
1
-
-
-
-
-
-
-
-
666036
Osawa
Crystal structure of the algin ...
Corynebacterium sp.
J. Mol. Biol.
345
1111-1118
2005
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1
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2
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-
666053
Yamasaki
A structural basis for depolym ...
Sphingomonas sp.
J. Mol. Biol.
352
11-21
2005
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1
8
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1
1
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3
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1
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8
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1
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663724
Trujillo-Roldan
The roles of oxygen and algina ...
Azotobacter vinelandii
Appl. Microbiol. Biotechnol.
63
742-747
2004
-
-
-
-
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1
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1
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2
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664518
Zhang
Preparation and structure eluc ...
Vibrio sp., Vibrio sp. 510
Carbohydr. Res.
339
1475-1481
2004
-
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3
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2
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664733
Han
Cloning, sequence analysis and ...
Vibrio sp., Vibrio sp. QY101
DNA Seq.
15
344-350
2004
-
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1
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1
1
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1
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6
-
1
1
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1
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1
1
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1
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1
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1
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1
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1
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1
1
1
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1
1
1
-
-
-
-
-
-
-
665371
Miyake
Origin and diversity of algina ...
Sphingomonas sp.
J. Bacteriol.
186
2891-2896
2004
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1
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1
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1
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4
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1
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1
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1
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1
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1
1
1
5
-
1
1
-
-
-
-
-
-
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665543
Yamasaki
Structure and function of a hy ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa AlgL
J. Biol. Chem.
279
31863-31872
2004
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1
1
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2
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5
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1
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2
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6
1
1
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1
2
1
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1
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1
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2
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1
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2
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6
1
1
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1
2
1
-
-
-
-
-
-
-
-
-
649405
Trujillo-Roldan
Alginate production by an Azot ...
Azotobacter vinelandii
Appl. Microbiol. Biotechnol.
60
733-737
2003
-
1
1
-
-
-
-
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1
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2
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2
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1
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1
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650644
Iwamoto
A study of tryptophan fluoresc ...
Pseudoalteromonas sp., Pseudoalteromonas sp. 272
Biosci. Biotechnol. Biochem.
67
1990-1992
2003
-
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5
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1
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15
-
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15
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
653039
Iwamoto
Primary structure and chemical ...
Pseudoalteromonas sp., Pseudoalteromonas sp. 272
J. Protein Chem.
21
455-463
2002
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1
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5
1
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1
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6
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4
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1
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5
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1
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1
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4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648533
Iwamoto
Purification and characterizat ...
Alteromonas sp., Alteromonas sp. 272
Biosci. Biotechnol. Biochem.
65
133-142
2001
-
-
-
-
-
-
4
12
1
4
2
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6
-
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1
1
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1
-
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21
1
1
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1
12
1
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1
-
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-
-
-
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4
-
12
1
4
2
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1
-
1
-
-
21
1
1
-
1
12
1
-
-
1
-
-
-
-
-
-
652156
Svanem
The catalytic activities of th ...
Azotobacter vinelandii
J. Biol. Chem.
276
31542-31550
2001
1
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1
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1
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1
1
1
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5
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1
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1
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3
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1
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2
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1
1
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1
-
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3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
652765
Yoon
-
Effect of His192 mutation on t ...
Sphingomonas sp.
J. Microbiol. Biotechnol.
11
118-123
2001
-
-
1
-
1
-
1
-
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1
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1
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1
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1
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1
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1
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-
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1
-
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-
-
-
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-
-
-
-
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-
-
-
-
652853
Yoon
Crystal structure of alginate ...
Sphingomonas sp.
J. Mol. Biol.
307
9-16
2001
-
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1
1
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-
-
-
-
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2
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1
1
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1
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1
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1
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
648532
Wong
Alginate lyase: review of majo ...
Alginovibrio aquatilis, Alteromonas sp. H-4, Aplysia depilans, Asteromyces cruciatus, Azotobacter chroococcum, Azotobacter chroococcum 4A1M, Azotobacter phage A22, Azotobacter phage A31, Azotobacter vinelandii, Azotobacter vinelandii phage, Bacillus circulans, Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) ATB-1015, Chlorella virus, Choromytilus meridionalis, Cobetia marina, Colpomenia sinuosa, Corollospora intermedia, Dollabella auricola, Eisenia bicyclis, Endarachne binghamiae, Fucus spp., Haliotis corrugata, Haliotis tuberculata, Ishige sp., Laminaria digitata, Littorina sp., Paradendryphiella arenariae, Paradendryphiella salina, Pelvetia canaliculata, Perna perna, Photobacterium sp., Photobacterium sp. ATCC 43367, Photobacterium sp. ATCC 43367 Alg-A, Pseudoalteromonas sp. IAM14594, Pseudomonas aeruginosa, Pseudomonas aeruginosa FRDI, Pseudomonas alginovora, Pseudomonas alginovora XO17, Pseudomonas putida, Pseudomonas syringae, Sargassum sagamianum, Spatoglossum pacificum, Sphingomonas sp., Spisula solidissima, Stenotrophomonas maltophilia, Turbo cornutus, uncultured bacterium, uncultured bacterium Alg-A, Undaria pinnatifida, Vibrio alginolyticus
Annu. Rev. Microbiol.
54
289-340
2000
8
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2
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1
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34
47
23
98
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55
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3
43
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18
30
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229
1
13
1
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30
-
-
-
-
12
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8
44
2
-
-
-
-
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1
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34
47
23
98
-
-
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3
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18
30
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229
1
13
1
-
-
30
-
-
12
-
-
-
-
-
-
650305
Yoon
Crystallization and preliminar ...
Sphingomonas sp.
Biochim. Biophys. Acta
1476
382-385
2000
-
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1
1
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1
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1
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1
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1
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1
-
-
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-
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-
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651661
Hashimoto
Molecular identification of ol ...
Sphingomonas sp.
J. Bacteriol.
182
4572-4577
2000
5
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1
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1
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1
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3
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5
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1
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1
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7
1
1
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1
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1
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5
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1
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1
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1
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2
3
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1
1
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1
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7
1
1
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1
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1
-
-
-
-
-
-
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651668
Preston
Characterization of alginate l ...
Pseudomonas syringae
J. Bacteriol.
182
6268-6271
2000
1
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1
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1
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1
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1
1
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1
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1
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1
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1
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1
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1
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2
1
1
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-
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1
-
-
-
-
-
-
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-
-
653728
Yoon
Overexpression in Escherichia ...
Sphingomonas sp.
Protein Expr. Purif.
19
84-90
2000
1
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1
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2
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4
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4
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1
1
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3
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2
1
3
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2
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2
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Cloning and expression of the ...
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Yoon
Crystal structure of alginate ...
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34064
Nakagawa
An effective method for isolat ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) ATB-1015
J. Appl. Microbiol.
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328-335
1998
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34065
Ertesvag
Biochemical properties and sub ...
Azotobacter vinelandii
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1998
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1
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2
2
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664973
Rehm
Alginate lyase from Pseudomona ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa CF1/M1
FEMS Microbiol. Lett.
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1998
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34063
Shimokawa
Purification and characterizat ...
Paradendryphiella salina
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61
636-640
1997
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Monday
Alginate synthesis in Pseudomo ...
Pseudomonas aeruginosa
J. bacteriol.
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625-632
1996
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34076
Fujiyama
Purification and characterizat ...
Pseudomonas sp., Pseudomonas sp. OS-ALG-9
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1995
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34077
Malissard
Overproduction and properties ...
uncultured marine bacterium
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1995
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698360
Lafay
Roseobacter algicola sp. nov., ...
no activity in Marinovum algicola, no activity in Marinovum algicola ATCC 51442
Int. J. Syst. Bacteriol.
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290-296
1995
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Boyd
Role of alginate lyase in cell ...
Pseudomonas aeruginosa
Appl. Environ. Microbiol.
60
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1994
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34078
Muramatsu
-
Characterization of alginate l ...
Pseudomonas sp.
Comp. Biochem. Physiol. B
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103-108
1990
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34075
Mramatsu
-
The role of sulfhydryl groups ...
Turbo cornutus
Agric. Biol. Chem.
51
1169-1171
1987
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34068
Romeo
-
Purification and structural pr ...
uncultured marine bacterium
Biochemistry
25
8385-8391
1986
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1
1
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34069
Romeo
-
Depolymerization of alginate b ...
uncultured marine bacterium
Biochemistry
25
8391-8396
1986
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2
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34070
Hansen
Distribution of alginate lyase ...
Bacillus circulans
Appl. Environ. Microbiol.
49
1019-1021
1985
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1
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1
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34061
Doubet
Properties of alginate lyases ...
uncultured marine bacterium
Appl. Environ. Microbiol.
47
699-703
1984
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2
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2
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1
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1
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2
1
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2
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2
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1
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1
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2
1
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34071
Muramatsu
-
Additional evidence for substr ...
Turbo cornutus
Agric. Biol. Chem.
48
811-813
1984
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1
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34072
Doubet
Isolation of marine bacteria c ...
Macrocystis pyrifera
Appl. Environ. Microbiol.
44
754-756
1982
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2
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34062
Maramatsu
-
Isozymes of alginate lyase in ...
Turbo cornutus
Agric. Biol. Chem.
44
2587-2594
1980
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1
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1
1
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1
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1
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2
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1
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1
1
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1
1
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1
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1
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34066
Muramatsu
-
A mode of action of alginate l ...
Turbo cornutus
Agric. Biol. Chem.
43
287-291
1979
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1
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34067
Muramatsu
-
Effect of EDTA on the purifica ...
Turbo cornutus
Agric. Biol. Chem.
43
2611-2612
1979
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1
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34073
Favorov
A study of the reaction cataly ...
Littorina sp.
Biochim. Biophys. Acta
569
259-266
1979
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2
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1
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2
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2
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2
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34060
Davidson
An alginate lysate from Azotob ...
Azotobacter vinelandii, Azotobacter vinelandii phage
J. Gen. Microbiol.
98
223-229
1977
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1
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4
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2
2
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1
1
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34074
Elyakova
-
Isolation and certain properti ...
Littorina sp.
Biochim. Biophys. Acta
358
341-354
1974
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1
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8
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2
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1
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6
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1
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8
1
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1
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2
1
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2
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1
1
2
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34080
Nisizawa
Alginate lyases in the hepatop ...
Dolabella auricularia
J. Biochem.
64
25-37
1968
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1
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34079
Nakada
Alginic acid degradation by el ...
Haliotis corrugata, Haliotis rufescens
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242
845-851
1967
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8
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