EC Number   |
Reference |
|---|
   4.1.1.2 | expression of functionally inactive OXDC in Escherichia coli, expression of active OXDC in Nicotiana tabaccum var. Petite Havanna and tomato plants var. Pusa Ruby in cytosol and vacuole |
652089 |
| 4.1.1.2 | gene FvOXDC, recombinant expression in Solanum lycopersicum fruits under the control of a fruit-specific promoter E8.2 being active upon ethylene induction only in ripening fruits, via Agrobacterium tumefaciens-mediated transformation, OXDC is targeted to the cell vacuole in the transgenic fruits. The transgenic fruits show up to a 90% reduction in oxalate content, which correlates with concomitant increases in calcium, iron, and citrate, compared to wild-type fruits. Expression of OXDC affects neither CO2 assimilation rates nor results in any detectable morphological differences in the transgenic plants. OXDC-responsive proteins involved in metabolism and stress responses represent the most substantially up- and down-regulated categories, respectively, in the transgenic fruit, compared with those of wild-type plants, proteome analysis, overview |
728524 |
| 4.1.1.2 | gene oxdC, cloning and expression in Escherichia coli strain DH5alpha and BL21(DE3), constitutive overexpression from plasmid pLB36, with promoter PldhL from Lactobacillus plantarum NCIMB8826, in Lactobacillus plantarum strain NC8. The recombinant Lactobacillus plantarum is able to degrade more than 90% of added oxalate, compared to 15% by the wild-type, and also shows higher tolerance to oxalate |
727707 |
| 4.1.1.2 | gene oxdC, cloning in Escherichia coli strain DH10B, functional overexpression of His-tagged enzyme in Lactobacillus plantarum strain WCFS1, isolated from human saliva, using homologous signal peptides Lp 0373 and Lp 3050, the recombinant strain secretes the recombinant protein. The recombinant Lactobacillus plantarum harboring pLp 0373sOxdC and pLp 3050sOxdC can express and secrete functional OxdC and degrade oxalate up to 50% and 30%, respectively |
727124 |
| 4.1.1.2 | gene oxdC, DNA and amino acid sequence determination and analysis, expression of the enzyme in transgenic lettuce, Lactuca sativa cv. Veronica, plants using Agrobacterium tumefaciens strain EHA 105-mediated transformation |
682396 |
| 4.1.1.2 | gene oxdC, expression of C-terminally His-tagged OxdC in Escherichia coli strain BL21(DE3) |
678138 |
| 4.1.1.2 | gene oxdC, expression of the C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
678229 |
| 4.1.1.2 | gene oxdC, expression of the C-terminally His6-tagged protein in Escherichia coli strain BL21(DE3) |
678075 |
| 4.1.1.2 | gene oxdC, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
677825 |
| 4.1.1.2 | oxdD gene, expression in Escherichia coli, genetic organization of the oxdD locus |
651732 |
