Cloned (Comment) | Organism |
---|---|
gene oxdC, expression of C-terminally His-tagged OxdC in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Fourier transform infrared spectroscopy to monitor in real time both substrate consumption and product formation, the Km for oxalate determined using this assay is 3.8fold lower than that estimated from a stopped assay, overview, solvent deuterium kinetic isotope effect, overview | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalate + H+ | Bacillus subtilis | - |
formate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene oxdC | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged OxdC from Escherichia coli strain BL21(DE3) | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
two assay methods, stopped assay and Fourier transform infrared spectroscopy to monitor in real time both substrate consumption and product formation | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also shows oxalate oxidase activity, catalytic cycle, overview | Bacillus subtilis | ? | - |
? | |
oxalate + H+ | - |
Bacillus subtilis | formate + CO2 | - |
? | |
oxalate + H+ | catalytic cycle, overview, the enzyme converts oxalate to formate and carbon dioxide, via an enzyme-bound formyl radical catalytic intermediate, and uses dioxygen as a cofactor despite the reaction involving no net redox change, overview, a proton transfer event occurs during a rate-limiting step, hydron exchange in formate, semiempirical quantum mechanical calculation, overview | Bacillus subtilis | formate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OXDC | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
stopped assay at | Bacillus subtilis |
26 | - |
Fourier transformation infrared spectroscopic assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
stopped assay at | Bacillus subtilis |
5 | - |
Fourier transformation infrared spectroscopic assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
O2 | - |
Bacillus subtilis |