EC Number   |
Reference |
|---|
   3.2.1.143 | gene ADPRHL2, recombinant expression of His-tagged human PARG catalytic domain (residues 448-976) from Escherichia coli strain Rosetta (DE3), recombinant expression of His-tagged full-length wild-type and mutant D314E enzymes in Escherichia coli strain BL21(DE3) |
754165 |
| 3.2.1.143 | gene ADPRHL2, sequence comparisons, recombinant expression of N-terminally His-tagged wild-type enzyme and GST-tagged mutant enzymes in Escherichia coli strain BL21(DE3) |
754168 |
| 3.2.1.143 | gene PARG, real-time PCR expression analysis |
753397 |
| 3.2.1.143 | gene PARG, recombinant expression of wild-type enzyme and catalytically inactive mutant E752N, both comprising residues 385-972, in Escherichia coli strain Tuner (DE3) cells, coexpression of GroESL chaperone |
754122 |
| 3.2.1.143 | gene PARG, single gene, recombinant expression of C-terminally His-tagged hPARG in Escherichia coli BL21(DE3) Gold |
752468 |
| 3.2.1.143 | gene PARG1, recombinant expression of GST-tagged wild-type and mutant enzymes |
755409 |
| 3.2.1.143 | phylogenetic analysis, expression of wild-type and selenomethionin-labeled enzymes in Escherichia coli strain Rosetta (DE3) |
732568 |
| 3.2.1.143 | recombinant expression of His6-tagged truncated enzyme comprising residues 385-972 in Escherichia coli strain BL21 co-expressing GroESL chaperone |
732567 |
| 3.2.1.143 | recombinant expression of wild-type and mutant enzymes |
754824 |
| 3.2.1.143 | the catalytic domain is expressed in Escherichia coli Rosetta2 (DE3) cells as a fusion protein with a glutathione S-transferase tag at the N-terminus and a hexahistidine tag at the C-terminus |
716831 |
