Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.143 extracted from

  • Zhang, H.; Gu, Z.; Wu, Q.; Yang, L.; Liu, C.; Ma, H.; Xia, Y.; Ge, X.
    Arabidopsis PARG1 is the key factor promoting cell survival among the enzymes regulating post-translational poly(ADP-ribosyl)ation (2015), Sci. Rep., 5, 15892 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.143

Cloned(Commentary)

Cloned (Comment) Organism
gene PARG1, recombinant expression of GST-tagged wild-type and mutant enzymes Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
E273N site-directed mutagenesis Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Arabidopsis thaliana 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly(ADP-ribose) + H2O Arabidopsis thaliana
-
 ?
-
 ?
poly(ADP-ribose) + H2O Arabidopsis thaliana Col-0
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SKB3
-
-
Arabidopsis thaliana Col-0 Q9SKB3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information PARG1 is induced primarily in mitotically active cells Arabidopsis thaliana
-
root apical meristem
-
 Arabidopsis thaliana
-
seedling
-
 Arabidopsis thaliana
-
shoot meristem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(ADP-ribose) + H2O
-
 Arabidopsis thaliana ?
-
 ?
poly(ADP-ribose) + H2O PARylated PARP1 substrate Arabidopsis thaliana ?
-
 ?
poly(ADP-ribose) + H2O
-
 Arabidopsis thaliana Col-0 ?
-
 ?
poly(ADP-ribose) + H2O PARylated PARP1 substrate Arabidopsis thaliana Col-0 ?
-
 ?

Synonyms

Synonyms Comment Organism
PARG1
-
 Arabidopsis thaliana
poly(ADP-ribose) glycohydrolase 1
-
 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana PARG1 expression is induced primarily in root and shoot meristems by bleomycin and induction of PARG1 is dependent on ATM and ATR kinases. PARG1 is induced primarily in mitotically active cells up

General Information

General Information Comment Organism
malfunction mutation of PARG1 results in increased DNA damage level and enhanced cell death in plants after bleomycin treatment. Inhibition or silencing of PARPs improves abiotic stress tolerance, enhancing resistance to drought, high light, heat and oxidative stresses, and perturbs innate immune responses to microbe-associated molecular patterns such as flg22 and elf18, resulting in a compromised basal defense response. Phenotypic comparison of the loss-of-function mutants of all PARP and PARG genes in Arabidopsis thaliana, overview. Loss-of-PARG1 leads to the transcriptional up-regulation of DNA repair genes and increase of cellular DNA damage level. The parg1 mutants show only yellow instead of green seedlings with reduced fresh weight compared to wild-type. The parg1-4 mutant root is more sensitive to bleomycin than that of wild-type Col-0 Arabidopsis thaliana
metabolism poly(ADP-ribosyl)ation is a reversible post-translational modification of proteins, characterized by the addition of poly(ADP-ribose) (PAR) to proteins by poly(ADP-ribose) polymerase (PARP), and removal of PAR by poly(ADP-ribose) glycohydrolase (PARG). Three PARPs and two PARGs have been found in Arabidopsis thaliana. PARG1 and PARG2 play an essential and a minor role, respectively under the same conditions Arabidopsis thaliana
physiological function PARG1 has poly(ADP-ribose) (PAR)-degrading activity and regulates poly(ADP-ribose) level in vivo. PARG1 and PARG2 play an essential and a minor role, respectively under the same conditions. PARG1 expression is induced primarily in root and shoot meristems by bleomycin and induction of PARG1 is dependent on ATM and ATR kinases. PARG1 antagonistically modulates the DNA repair process by preventing the over-induction of DNA repair genes. PARG1 plays a critical role in this process. Roles of PARP1 and PARP2 in DNA damage signaling. Induction of PARG1 gene is ATM- and ATR-dependent and PARG1 represses the transcriptional upregulation of ATM, ATR and SOG1. ATM and ATR are two critical kinases which transduce double and single strand break signals to DNA repair machinery, respectively. They phosphorylate the transcription factor SOG1, which then induces the expression of DNA repair genes Arabidopsis thaliana