3.2.1.143 cloning from genomic DNA, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), quantitative RT-PCR expression analysis 752519 3.2.1.143 expressed in Escherichia coli 716339 3.2.1.143 expressed in Escherichia coli K12 TB1 and Rosetta ells 714215 3.2.1.143 expression as glutathione S-transferase fusion protein 677955 3.2.1.143 expression in Escherichia coli 208516 3.2.1.143 expression in Escherichia coli as His-tagged protein 679467 3.2.1.143 expression in HeLa cell 680710 3.2.1.143 expression of His-tagged wild-type and mutant enzymes and selenomethionine-labeled enzyme in Escherichia coli strain BL21 (DE3) GOLD 732714 3.2.1.143 expression of isoform PARG59 in HeLa cell 708414 3.2.1.143 fusion to enhanced green fluorescent protein and expression in Drosophila melanogaster 680021 3.2.1.143 gene ADPRHL2, recombinant expression of His-tagged human PARG catalytic domain (residues 448-976) from Escherichia coli strain Rosetta (DE3), recombinant expression of His-tagged full-length wild-type and mutant D314E enzymes in Escherichia coli strain BL21(DE3) 754165 3.2.1.143 gene ADPRHL2, sequence comparisons, recombinant expression of N-terminally His-tagged wild-type enzyme and GST-tagged mutant enzymes in Escherichia coli strain BL21(DE3) 754168 3.2.1.143 gene PARG, real-time PCR expression analysis 753397 3.2.1.143 gene PARG, recombinant expression of wild-type enzyme and catalytically inactive mutant E752N, both comprising residues 385-972, in Escherichia coli strain Tuner (DE3) cells, coexpression of GroESL chaperone 754122 3.2.1.143 gene PARG, single gene, recombinant expression of C-terminally His-tagged hPARG in Escherichia coli BL21(DE3) Gold 752468 3.2.1.143 gene PARG1, recombinant expression of GST-tagged wild-type and mutant enzymes 755409 3.2.1.143 phylogenetic analysis, expression of wild-type and selenomethionin-labeled enzymes in Escherichia coli strain Rosetta (DE3) 732568 3.2.1.143 recombinant expression of His6-tagged truncated enzyme comprising residues 385-972 in Escherichia coli strain BL21 co-expressing GroESL chaperone 732567 3.2.1.143 recombinant expression of wild-type and mutant enzymes 754824 3.2.1.143 the catalytic domain is expressed in Escherichia coli Rosetta2 (DE3) cells as a fusion protein with a glutathione S-transferase tag at the N-terminus and a hexahistidine tag at the C-terminus 716831