EC Number   |
Reference |
|---|
    2.7.7.43 | high-level expression in Escherichia coli |
645266 |
| 2.7.7.43 | human CMP-N-acetylneuraminic acid synthetase is expressed in tobacco BY2 suspension-cultured cells, enzyme is demonstrated to be active |
671731 |
| 2.7.7.43 | the enzyme is subcloned for overexpression in Escherichia coli K12 using the expression vector pKK233-3 |
645279 |
| 2.7.7.43 | the gene is cloned into a T7 expression vector, the protein is expressed in Escherichia coli |
645274 |
| 2.7.7.43 | the N-terminal domain, residues 39-267, and the C-terminal domain, residues 267-432, of CMP-sialic acid synthetase, and CMP-sialic acid synthetase, residues 39-432, are cloned into a pET22b-Strep vector |
705163 |
| 2.7.7.43 | transfection of NIH-3T3 and EPC cells |
722748 |
| 2.7.7.43 | transformation of the CMP-NeuNAc defective Escherichia coli K1 strain EV5 with CMP-NeuNAc synthetase from Neisseria meningitidis can complement the defect in Escherichia coli |
645259 |
