EC Number   |
|---|
   2.7.11.7 | expression of GFP-tagged isozyme MHCK A in a Dictyostelium discoideum strain lacking endogenous MHCK A, expression of a truncation mutant DELTAcoil-MHCK A comprising residues 1-498 in Escherichia coli as GST-fused protein |
| 2.7.11.7 | expression of MHCK A, comprising residues 527-887, i.e. A-CAT, as a GST-fusion protein, phylogenetic analysis |
| 2.7.11.7 | expression of MHCK B, comprising residues 13-459, i.e. B-CAT, as a GST-fusion protein, phylogenetic analysis |
| 2.7.11.7 | expression of the 3 isozymes MHCK A, MHCK B, and MHCK C as GFP-fusion proteins |
| 2.7.11.7 | expression of the catalytic domain of MHCK B as a GST-fusion protein in Escherichia coli BL21(DE3) |
| 2.7.11.7 | expression of the His-tagged catalytic domain of MHCK A in Escherichia coli BL21(DE3) |
| 2.7.11.7 | expression of the His-tagged catalytic domain of MHCK C in Escherichia coli BL21(DE3) |
| 2.7.11.7 | gene mhck A, construction of overexpressing cell line by transfection of an expression plasmid into the deficient mhck A- cell line |
| 2.7.11.7 | MHCK B, overexpression as FLAG-tagged protein in AX2 cells or 3xALA cells of Dictyostelium discoideum |
| 2.7.11.7 | overexpression of FLAG-tagged full-length MHCK-B in Dictyostelium cells, in AX2, mhkB-null, and mhkA/B/C-null cell lines. Expression of GST-tagged wild-type full-length and MHCKB truncation mutants |
