2.7.11.7	-	
2.7.11.7	cells overexpressing GFP-GEF have both unphosphorylated and autophosphorylated MHCK A	
2.7.11.7	cloning of wild-type and mutants of the catalytic domain of myosin I heavy chain kinase as His-tagged proteins and expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system	
2.7.11.7	construction of His-tagged or FLAG-tagged N-terminal enzyme peptides by site directed mutagenesis for introduction of start and stop codons, expression in Escherichia coli BL21(DE3)	
2.7.11.7	construction of His-tagged or GFP fusion proteins with the full length enzyme or enzyme fragments, expression in Dictyostelium discoideum cells	
2.7.11.7	DNA and amino acid sequence determination and analysis	
2.7.11.7	DNA sequence determination and analysis	
2.7.11.7	DNA sequence determination and analysis, gene MHCK possesses all of the domains characteristic of members of the protein kinase C family	
2.7.11.7	expressed in Escherichia coli BL21(DE3) cells	
2.7.11.7	expression of FLAG-tagged T825A and T825E enzyme mutants in Dictyostelium discoideum cells, expression of His-tagged wild-type and mutant MHCK A alpha-kinase domains in Escherichia coli strain BL21(DE3)	
2.7.11.7	expression of GFP-tagged isozyme MHCK A in a Dictyostelium discoideum strain lacking endogenous MHCK A, expression of a truncation mutant DELTAcoil-MHCK A comprising residues 1-498 in Escherichia coli as GST-fused protein	
2.7.11.7	expression of MHCK A, comprising residues 527-887, i.e. A-CAT, as a GST-fusion protein, phylogenetic analysis	
2.7.11.7	expression of MHCK B, comprising residues 13-459, i.e. B-CAT, as a GST-fusion protein, phylogenetic analysis	
2.7.11.7	expression of the 3 isozymes MHCK A, MHCK B, and MHCK C as GFP-fusion proteins	
2.7.11.7	expression of the catalytic domain of MHCK B as a GST-fusion protein in Escherichia coli BL21(DE3)	
2.7.11.7	expression of the His-tagged catalytic domain of MHCK A in Escherichia coli BL21(DE3)	
2.7.11.7	expression of the His-tagged catalytic domain of MHCK C in Escherichia coli BL21(DE3)	
2.7.11.7	gene mhck A, construction of overexpressing cell line by transfection of an expression plasmid into the deficient mhck A- cell line	
2.7.11.7	MHCK B, overexpression as FLAG-tagged protein in AX2 cells or 3xALA cells of Dictyostelium discoideum	
2.7.11.7	overexpression of FLAG-tagged full-length MHCK-B in Dictyostelium cells, in AX2, mhkB-null, and mhkA/B/C-null cell lines. Expression of GST-tagged wild-type full-length and MHCKB truncation mutants	
2.7.11.7	overexpression of His-tagged full-length isozyme MHCK A and truncation mutant DELTAcoil-MHCK A comprising residues 1-498 in Dictyostelium discoideum, overexpression of isolated coiled-coil domain and of the catalytic domain in Escherichia coli as GST-fused proteins	
2.7.11.7	subcloning and expression in Escherichia coli, expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system