EC Number   |
|---|
   1.1.1.10 | expression of wild-type and mutant enzymes in Escherichia coli |
| 1.1.1.10 | expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
| 1.1.1.10 | gene dhs-21, DNA and amino acid sequence determination and analysis, the dhs-21 gene is physically mapped to the cosmid R11D1 between the sma-1 and mes-4genes on chromosome V, expression of His6-tagged enzyme. Spatial and temporal expression pattern of the DHS-21-GFP construct in transgenic worms, overview |
| 1.1.1.10 | gene lxr3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Escherichia coli strain BL21(DE3) |
| 1.1.1.10 | gene lxr3, phylogenetic analysis, cloning in Escherichia coli strain JM109, expression of N-terminal His-tagged in Saccharomyces cerevisiae strain CEN.PK2-1D |
| 1.1.1.10 | gene xr, recombinant expression in Escherichia coli strain W3110 from p43-XR comprising a constitutive promotor and gene xr, subcloning in Escherichia coli strain DH5alpha, gene xr expression analysis by quantitative RT-PCR |
| 1.1.1.10 | recombinant overexpression of strictly NADPH-dependent XR with mutated strictly NADP+-dependent XDH leading to increased bioethanol production and decreased xylitol accumulation in Saccharomyces cerevisiae strain D452-2 resulting in mutant strains SK-N1 to SK-N4 |
| 1.1.1.10 | Tn5 transposon transfer |
| 1.1.1.10 | transgenic mice over-expressing dicarbonyl/L-xylulose reductase gene crossed with KK-A(y) diabetic model mice serve as an animal model for the metabolism of renal carbonyl compounds |
