EC Number   |
Substrates |
Organism |
Products |
Reversibility  |
|---|
    3.5.5.7 | more |
no activity with mandelonitrile, glycolonitrile |
Thermotoga maritima |
? |
- |
- |
| 3.5.5.7 | more |
the enzyme shows a broad hydrolytic activity toward aliphatic and heterocyclic nitriles and shows high tolerance of 3-cyanopyridine. No activity with: benzonitrile, phenylacetonitrile, 4-chlorobenzyl cyanide, mandelonitrile, 2-chloromandelonitrile, alpha-methylphenylacetonitrile, 1,2-phenylenediacetonitrile, dodecanenitrile |
Cupriavidus necator |
? |
- |
- |
| 3.5.5.7 | more |
the enzyme shows broad substrate specificity towards aliphatic, aromatic, and heterocyclic nitriles |
Pannonibacter carbonis |
? |
- |
- |
| 3.5.5.7 | more |
the enzyme shows preference for unsaturated aliphatic substrates containing 5-6 carbon atoms. Increased reaction rates are also found for aliphatic nitriles carrying electron withdrawing substituents (e.g. chloro- or hydroxy-groups) close to the nitrile group. Aliphatic dinitriles are attacked only at one of the nitrile groups and with most of the tested dinitriles the monocarboxylates are detected as major products. Substrates converted with 1% less compared to the activity with 3-hexenedinitrile: 2-methylbutyronitrile, decanedinitrile, octanedinitrile, cis,trans-crotononitrile, popionitrile, isovaleronitrile, lactonitrile, butyronitrile |
Pseudomonas fluorescens |
? |
- |
- |
| 3.5.5.7 | more |
the enzyme shows broad substrate specificity towards aliphatic, aromatic, and heterocyclic nitriles |
Pannonibacter carbonis Q4.6 |
? |
- |
- |
| 3.5.5.7 | (E)-2-methyl-2-butenenitrile + H2O |
regioselective hydrolysis, no detectable conversion of (Z)-2-methyl-2-butenenitrile |
Acidovorax facilis |
(E)-2-methyl-2-butenoic acid + NH3 |
- |
? |
| 3.5.5.7 | (E)-2-methyl-2-butenenitrile + H2O |
regioselective hydrolysis, no detectable conversion of (Z)-2-methyl-2-butenenitrile |
Acidovorax facilis 72W |
(E)-2-methyl-2-butenoic acid + NH3 |
- |
? |
| 3.5.5.7 | 1,4-dicyano-2-butene + H2O |
42.6% of the activity with crotononitrile |
Rhodococcus rhodochrous |
? |
- |
? |
| 3.5.5.7 | 1,4-dicyanobutane + H2O |
114.3% activity compared to 3-cyanopyridine |
Cereibacter sphaeroides |
? |
- |
? |
| 3.5.5.7 | 1,8-dicyanooctane + H2O |
114.3% activity compared to 3-cyanopyridine |
Cereibacter sphaeroides |
? |
- |
? |
