EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
6.2.1.54 | ATP + D-alanine + holo-[D-alaninyl-carrier protein] |
- |
Bacillus cereus DSM 31 |
AMP + diphosphate + D-alanyl-[D-alanyl-carrier protein] |
- |
? |
6.2.1.54 | ATP + D-alanine + poly(ribitol phosphate) |
- |
Bacillus cereus |
AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate) |
- |
? |
6.2.1.54 | ATP + D-alanine + poly(ribitol phosphate) |
the biosynthesis of D-alanyl-lipoteichoic acid requires four proteins that are encoded by the dlt operon. The synthesis starts with the selection of the D-Ala by the 57000 Da D-Ala-D-Ala carrier protein ligase (DltA). Following activation by DltA, D-Ala is transferred to the 10000 Da D-alanyl carrier protein DltC which can donate D-Ala to lipoteichoic acids with the help of DltB and DltD to mediate the surface charge of the bacterium |
Bacillus subtilis |
AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate) |
- |
? |
6.2.1.54 | ATP + D-alanine + poly(ribitol phosphate) |
- |
Bacillus cereus DSM 31 |
AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate) |
- |
? |
6.2.1.54 | ATP + D-Ser + diphosphate |
activity and specificity of DltA is investigated using the ATP-pyrophosphate exchange assay. Enzyme shows only minor activity 13% with D-Ser |
Bacillus subtilis |
? |
- |
? |
6.2.1.54 | ATP + L-Ala + diphosphate |
- |
Bacillus cereus |
? |
- |
? |
6.2.1.54 | ATP + L-alanine + holo-[D-alaninyl-carrier protein] |
- |
Bacillus cereus |
AMP + diphosphate + L-alanyl-[D-alanyl-carrier protein] |
- |
? |
6.2.1.54 | ATP + L-alanine + poly(ribitol phosphate) |
low activity |
Bacillus cereus |
AMP + diphosphate + O-L-alanyl-poly(ribitol phosphate) |
- |
? |
6.2.1.54 | D-Alanine + ATP + ? |
biosynthesis of D-alanyl-lipoteichoic acid |
Lacticaseibacillus casei |
? |
- |
? |
6.2.1.54 | more |
the enzyme is required for incorporation of D-alanine in teichoic acids in Gram-positive bacteria, confering resistance to cationic antimicrobial peptides nisin and gallidermin in Streptococcus pneumoniae, enzymes of D-alanine metabolism are encoded in the dltA operon, a functional dlt operon confers resistance to nisin and gallidermin |
Streptococcus pneumoniae |
? |
- |
? |