6.2.1.54	(D-alanyl)adenylate + holo-[D-alanyl-carrier protein]	-	Bacillus cereus	AMP + D-alanyl-[D-alanyl-carrier protein]	-	?	442656	
6.2.1.54	(D-alanyl)adenylate + holo-[D-alanyl-carrier protein]	-	Bacillus cereus DSM 31	AMP + D-alanyl-[D-alanyl-carrier protein]	-	?	442656	
6.2.1.54	ATP + D-2-amino butyric acid + diphosphate	activity and specificity of DltA is investigated using the ATP-pyrophosphate exchange assay. Enzyme shows only minor activity 13% with D-amino butyric acid	Bacillus subtilis	?	-	?	400123	
6.2.1.54	ATP + D-Ala + acyl-carrier protein	acyl carrier protein of E. coli, Vibrio harveyi, Saccharopolyspora erythraea, not of Bacillus subtilis, Spinacia oleracea	Lacticaseibacillus casei	D-Alanyl-acyl-carrier protein + AMP + diphosphate	-	?	141675	
6.2.1.54	ATP + D-Ala + D-alanyl carrier protein	-	Lacticaseibacillus casei	D-Alanyl-D-alanyl carrier protein + AMP + diphosphate	-	?	141670	
6.2.1.54	ATP + D-Ala + diphosphate	-	Bacillus cereus	?	-	?	400126	
6.2.1.54	ATP + D-Ala + diphosphate	activity and specificity of DltA is investigated using the ATP-pyrophosphate exchange assay. Enzyme is highly specific for cognate D-Ala substrate	Bacillus subtilis	?	-	?	400126	
6.2.1.54	ATP + D-alanine	-	Bacillus cereus	(D-alanyl)adenylate + diphosphate	-	?	443128	
6.2.1.54	ATP + D-alanine	-	Bacillus cereus DSM 31	(D-alanyl)adenylate + diphosphate	-	?	443128	
6.2.1.54	ATP + D-alanine + holo-[D-alaninyl-carrier protein]	-	Bacillus cereus	AMP + diphosphate + D-alanyl-[D-alanyl-carrier protein]	-	?	443127	
6.2.1.54	ATP + D-alanine + holo-[D-alaninyl-carrier protein]	-	Bacillus cereus DSM 31	AMP + diphosphate + D-alanyl-[D-alanyl-carrier protein]	-	?	443127	
6.2.1.54	ATP + D-alanine + poly(ribitol phosphate)	-	Bacillus cereus	AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate)	-	?	368865	
6.2.1.54	ATP + D-alanine + poly(ribitol phosphate)	the biosynthesis of D-alanyl-lipoteichoic acid requires four proteins that are encoded by the dlt operon. The synthesis starts with the selection of the D-Ala by the 57000 Da D-Ala-D-Ala carrier protein ligase (DltA). Following activation by DltA, D-Ala is transferred to the 10000 Da D-alanyl carrier protein DltC which can donate D-Ala to lipoteichoic acids with the help of DltB and DltD to mediate the surface charge of the bacterium	Bacillus subtilis	AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate)	-	?	368865	
6.2.1.54	ATP + D-alanine + poly(ribitol phosphate)	-	Bacillus cereus DSM 31	AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate)	-	?	368865	
6.2.1.54	ATP + D-Ser + diphosphate	activity and specificity of DltA is investigated using the ATP-pyrophosphate exchange assay. Enzyme shows only minor activity 13% with D-Ser	Bacillus subtilis	?	-	?	400127	
6.2.1.54	ATP + L-Ala + diphosphate	-	Bacillus cereus	?	-	?	400196	
6.2.1.54	ATP + L-alanine + holo-[D-alaninyl-carrier protein]	-	Bacillus cereus	AMP + diphosphate + L-alanyl-[D-alanyl-carrier protein]	-	?	443147	
6.2.1.54	ATP + L-alanine + poly(ribitol phosphate)	low activity	Bacillus cereus	AMP + diphosphate + O-L-alanyl-poly(ribitol phosphate)	-	?	443148	
6.2.1.54	D-Alanine + ATP + ?	biosynthesis of D-alanyl-lipoteichoic acid	Lacticaseibacillus casei	?	-	?	369904	
6.2.1.54	additional information	the enzyme is required for incorporation of D-alanine in teichoic acids in Gram-positive bacteria, confering resistance to cationic antimicrobial peptides nisin and gallidermin in Streptococcus pneumoniae, enzymes of D-alanine metabolism are encoded in the dltA operon, a functional dlt operon confers resistance to nisin and gallidermin	Streptococcus pneumoniae	?	-	?	89	
6.2.1.54	additional information	the ATP and D-alanine substrates of the adenylation reaction are incorporated when the DltA enzyme cycles through its thiolation conformation	Bacillus cereus	?	-	?	89	
6.2.1.54	additional information	the ATP and D-alanine substrates of the adenylation reaction are incorporated when the DltA enzyme cycles through its thiolation conformation	Bacillus cereus DSM 31	?	-	?	89